Overview

  • Product nameAnti-Bcl2 alpha antibody [8C8] (Biotin)
    See all Bcl2 alpha primary antibodies
  • Description
    Mouse monoclonal [8C8] to Bcl2 alpha (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Monkey
    Predicted to work with: Cow, Cat, Dog, PigDoes not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    C-GAAPAPGIFSSQPG

    , corresponding to amino acids 41-54 of Human Bcl2 alpha

  • Positive control
    • Raji or HeLa Cell lysates. Tonsil or follicular lymphoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab79204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 - 2 µg/ml for 30 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
    WB: Use at a concentration of 1 µg/ml for 2 hours at room temperature. Predicted molecular weight: 26 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
    • Cellular localizationMitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
    • Database links
    • Alternative names
      • Apoptosis regulator Bcl2 antibody
      • B cell lymphoma protein 2 alpha isoform antibody
      • B cell lymphoma protein 2 antibody
      • B-cell CLL/lymphoma 2 antibody
      • B-cell leukemia/lymphoma 2 antibody
      • Bcl 2 antibody
      • Leukemia /lymphoma, B-cell, 2 antibody
      • Oncogene B-cell leukemia 2 antibody
      see all

    References for Anti-Bcl2 alpha antibody [8C8] (Biotin) (ab79204)

    This product has been referenced in:
    • Wolter KG  et al. Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 139:1281-92 (1997). WB, IF ; Monkey . Read more (PubMed: 9382873) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"