The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use 1-2µl per IP from 10-50 million cells.
WB: 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Tested on transfected cells and endogenous protein in various lympoid cell lines.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRetards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection.
Tissue specificitySeems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies.