• Product nameAnti-Bcl2A1 antibody [SPM117]
    See all Bcl2A1 primary antibodies
  • Description
    Mouse monoclonal [SPM117] to Bcl2A1
  • Tested applicationsSuitable for: WB, IP, ELISA, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence within amino acids 40-55 of Human Bcl2A1.

  • Positive control
    • Raji whole cell lysate; Human tonsil tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: Malme-3M


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Constituent: 99% Tris buffered saline
    Note: contains antibody stabilizer
  • Concentration information loading...
  • PurityAscites
  • ClonalityMonoclonal
  • Clone numberSPM117
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab125259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 20 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionRetards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection.
  • Tissue specificitySeems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACC 1 antibody
    • ACC 2 antibody
    • B2LA1_HUMAN antibody
    • Bcl 2 related protein A1 antibody
    • Bcl-2-like protein 5 antibody
    • Bcl-2-related protein A1 antibody
    • BCL2 related protein A1 antibody
    • Bcl2-L-5 antibody
    • BCL2A1 antibody
    • BCL2L5 antibody
    • BFL1 antibody
    • GRS antibody
    • HBPA1 antibody
    • Hematopoietic BCL2 related protein A1 antibody
    • Hemopoietic specific early response protein antibody
    • Hemopoietic-specific early response protein antibody
    • Protein BFL 1 antibody
    • Protein BFL-1 antibody
    • Protein GRS antibody
    see all

Anti-Bcl2A1 antibody [SPM117] images

  • ICC/IF image of ab125259 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab125259 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was a goat anti-mosue Alexa Fluor® 488 (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Overlay histogram showing HeLa cells stained with ab125259 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125259, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Anti-Bcl2A1 antibody [SPM117] (ab125259) at 1/500 dilution + Raji whole cell lysate

    Predicted band size : 20 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    12% SDS-PAGE.
  • ab125259 at 1/25 dilution ,staining Bcl2A1 in formalin-fixed paraffin-embedded Human tonsil tissue, by Immunohistochemistry.

References for Anti-Bcl2A1 antibody [SPM117] (ab125259)

ab125259 has not yet been referenced specifically in any publications.

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