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Western blot - Bcl xL antibody (ab2568)
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Immunoprecipitation - Bcl xL antibody (ab2568)
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Western blot - Bcl xL antibody (ab2568)
(enlarge)
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Product Name
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BCL2L1 antibody
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See all BCL2L1 antibodies (32)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BCL2L1
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Immunogen
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Synthetic peptide surrounding amino acid 56 of human Bcl xL.
(Peptide available as ab52114.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody detects the 30 kDa Bcl-xL protein. It does not react with other Bcl-2 family members.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 20 µg/ml.
WB: Use at a concentration of 1.0 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Mitochondrial membranes and perinuclear envelope
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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The protein encoded by this gene belongs to the Bcl2 protein family. Bcl2 family members form hetero or homodimers and act as anti or pro apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BCL2L1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BCL2L1 antibody images, references, products related to ab2568 and other tools.
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BCL2L1 antibody images:
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 Western blot - Bcl xL antibody (ab2568)
BCL2L1 antibody (ab2568) at 1/1000 dilution + 25 ug HeLa whole cell lysate at 25 µg
Secondary
Goat anti rabbit (HRP) at 1/4000 dilution
Performed under reducing conditions.
This image was submitted as part of an anonymous Abreview received on the 30th September 2005. We do not have any additional information relating to this image.
See Abreview
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Immunoprecipitation - Bcl xL antibody (ab2568)
ab2568 used to immunoprecipitate Bcl xL from whole cell lysate of a human Retinal Pigment Epithelial cell line. 7.5ug of antibody was added per mg of lysate and incubated together with Protein A for 1 hour. 500ug of lysate was added per lane. A peroxidase conjugated goat anti-rabbit IgG was used as the secondary antibody. Lane 1: Immunoprecipitated material Lane 2: Control lane (no antibody) This image is courtesy of an Abreview submitted by Ingram Iaccarino on 12 April 2006
See Abreview
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Western blot - Bcl xL antibody (ab2568)
All lanes : BCL2L1 antibody (ab2568) at 1/1000 dilution
Lane 1 : 20ug whole cell lysate from Retinal Pigment Epithelia
Lane 2 : 20ug whole cell lysate from MDA MB231 cells
Lane 3 : 20ug whole cell lysate from HeLa cells
Secondary
Goat anti-rabbit HRP
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
This image is courtesy of an Abreview submitted by Ingram Iaccarino.
See Abreview
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References for BCL2L1 antibody (ab2568)
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This product has been used in: (two most recent references)
Zimmerman MC et al. Mutant SOD1-induced neuronal toxicity is mediated by increased mitochondrial superoxide levels. J Neurochem 102:609-18 (2007). PubMed: 17394531
Paduano F et al. Silencing of survivin gene by small interfering RNAs produces supra-additive growth suppression in combination with 17-allylamino-17-demethoxygeldanamycin in human prostate cancer cells. Mol Cancer Ther 5:179-86 (2006). PubMed: 16432177
See all 4 publication references for this product.
If you publish research using ab2568 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to BCL2L1
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BCL2L1 antibody - more information
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Customer reviews (feedback) regarding BCL2L1 antibody |
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BCL2L1 antibody - related products:
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BCL2L1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BCL2L1 antibody |
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