Overview

  • Product nameAnti-Bcl2L2 antibody
    See all Bcl2L2 primary antibodies
  • Description
    Rabbit polyclonal to Bcl2L2
  • Tested applicationsSuitable for: IHC-FoFr, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    This antibody was developed against a synthetic peptide corresponding within amino acid residues 1-100 of human Bcl2L2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 21289171
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 21 kDa.
IHC-P 1/50 - 1/100.

Target

  • FunctionPromotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
  • Tissue specificityExpressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainThe BH4 motif seems to be involved in the anti-apoptotic function.
    The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2.
  • Cellular localizationMitochondrion membrane. Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator BCL W antibody
    • Apoptosis regulator Bcl-W antibody
    • B2CL2_HUMAN antibody
    • BCL 2 Like 2 antibody
    • Bcl 2 like 2 protein antibody
    • Bcl 2L2 antibody
    • BCL W antibody
    • Bcl-2-like protein 2 antibody
    • Bcl2 L2 antibody
    • BCL2 like 2 antibody
    • BCL2 like 2 protein antibody
    • Bcl2-L-2 antibody
    • Bcl2l2 antibody
    • BCLW antibody
    • KIAA0271 antibody
    • PPP1R51 antibody
    • Protein phosphatase 1 regulatory subunit 51 antibody
    see all

Anti-Bcl2L2 antibody images

  • Anti-Bcl2L2 antibody (ab38629) + HL-60 cell lysate

    Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References for Anti-Bcl2L2 antibody (ab38629)

This product has been referenced in:
  • Ibrahim MY  et al. a-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-?B and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther 8:1629-47 (2014). WB ; Human . Read more (PubMed: 25302018) »
  • Ibrahim MY  et al. Involvement of NF-?B and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, a-mangostin, from Cratoxylum arborescens. Drug Des Devel Ther 8:2193-211 (2014). WB ; Human . Read more (PubMed: 25395836) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"