• Product name
  • Description
    Rabbit polyclonal to Bcl3
  • Tested applications
    Suitable for: WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) - mapping to the C terminus of Bcl 3.



Our Abpromise guarantee covers the use of ab27780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
IP Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use a concentration of 20 - 40 µg/ml.


  • Function
    Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
  • Involvement in disease
    Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
  • Sequence similarities
    Contains 7 ANK repeats.
  • Post-translational
    Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
    Activated by phosphorylation.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell CLL/lymphoma 3 antibody
    • B cell leukemia/lymphoma 3 antibody
    • B cell lymphoma 3 encoded protein antibody
    • B-cell lymphoma 3 protein antibody
    • Bcl 3 antibody
    • BCL 4 antibody
    • BCL-3 antibody
    • Bcl3 antibody
    • BCL3_HUMAN antibody
    • BCL4 antibody
    • Chronic lymphatic leukemia protein antibody
    • D19S37 antibody
    • Protein Bcl 3 antibody
    • Proto-oncogene BCL3 antibody
    see all

Anti-Bcl3 antibody images

  • Anti-Bcl3 antibody (ab27780) at 2 µg/ml + Jurkat

    Predicted band size : 47 kDa

References for Anti-Bcl3 antibody (ab27780)

This product has been referenced in:
  • Long TY  et al. CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-?B pathway. Braz J Med Biol Res 50:e5861 (2017). Read more (PubMed: 28355355) »

See 1 Publication for this product

Product Wall

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (pigmented skin lessions)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EnVision Flex hight Dako
pigmented skin lessions
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 37°C

Abcam user community

Verified customer

Submitted Mar 22 2017

Western blot
Human Cell lysate - whole cell (Colon)
Loading amount
80 µg
Basal and Knockdown (siRNA)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 23 2012

Thank you very much for that feedback.

I am pleased to hear that the anti-Bcl3 antibody ab49470 is showing promise in your experiments. This information would be very valuable to other customers so I would be very interested to hear when you ...

Read More

That is no problem at all, we would be happy to receive the feedback whenever you are ready to give it.

I have now arranged for the two antibodies we discussed to be sent to you (free of charge). The antibody ab13708 is however not directly i...

Read More

Thank you for getting back to me.

I can provide you any antibody which you would prefer. Ab13708 is marginally more popular but not by much. I will also check if we have any information on if the mouse monoclonal ab49470 is likely to work in ...

Read More

Sorry for the delay in getting back to you.

I have checked the QC data for the particular lot of ab27780 you have been using and it looks as clean as the Western blot presented on the datasheet of this product using Jurkat cell lysate. One th...

Read More

Thank you for calling us yesterday and for alerting us to the problem you are experiencing with theAnti-Bcl3 antibody (ab27780) and sorry for the delay in getting back to you. We take product complaints very seriously, and investigate every product tha...

Read More

Thank you for your email.

I am pleased to hear that the ab49470 worked for you. I am currently seeking information as to whether we have a further 5 vials of the lot you tried in stock. I will get back to you with this information as soon as I...

Read More


Sign up