Overview

  • Product name
  • Description
    Rabbit polyclonal to Bcl3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) - mapping to the C terminus of Bcl 3.

Properties

Applications

Our Abpromise guarantee covers the use of ab27780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
IP Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use a concentration of 20 - 40 µg/ml.

Target

  • Function
    Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
  • Involvement in disease
    Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
  • Sequence similarities
    Contains 7 ANK repeats.
  • Post-translational
    modifications
    Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
    Activated by phosphorylation.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell CLL/lymphoma 3 antibody
    • B cell leukemia/lymphoma 3 antibody
    • B cell lymphoma 3 encoded protein antibody
    • B-cell lymphoma 3 protein antibody
    • Bcl 3 antibody
    • BCL 4 antibody
    • BCL-3 antibody
    • Bcl3 antibody
    • BCL3_HUMAN antibody
    • BCL4 antibody
    • Chronic lymphatic leukemia protein antibody
    • D19S37 antibody
    • Protein Bcl 3 antibody
    • Proto-oncogene BCL3 antibody
    see all

Images

  • Anti-Bcl3 antibody (ab27780) at 2 µg/ml + Jurkat

    Predicted band size: 47 kDa

References

This product has been referenced in:
  • Long TY  et al. CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-?B pathway. Braz J Med Biol Res 50:e5861 (2017). Read more (PubMed: 28355355) »

See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pigmented skin lessions)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EnVision Flex hight Dako
Permeabilization
No
Specification
pigmented skin lessions
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 37°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 22 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Colon)
Loading amount
80 µg
Specification
Colon
Treatment
Basal and Knockdown (siRNA)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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