Overview

  • Product nameAnti-Bcl6 antibody
    See all Bcl6 primary antibodies
  • Description
    Rabbit polyclonal to Bcl6
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Bcl6.

    (Peptide available as ab97495.)

  • Positive control
    • This antibody gave a positive signal in Mouse and Rat Brain tissue lysate as well as the following whole cell lysates: Jurkat; Ramos; K562.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).
IP Use a concentration of 5 µg/ml.

Target

  • FunctionTranscriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis.
  • Tissue specificityExpressed in germinal center T and B cells and in primary immature dendritic cells.
  • Involvement in diseaseNote=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
    Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
    Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF.
  • Sequence similaritiesContains 1 BTB (POZ) domain.
    Contains 6 C2H2-type zinc fingers.
  • DomainThe BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2.
  • Post-translational
    modifications
    Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell CLL/lymphoma 6 antibody
    • B cell lymphoma 6 protein antibody
    • B-cell lymphoma 5 protein antibody
    • B-cell lymphoma 6 protein antibody
    • BCL 5 antibody
    • Bcl 6 antibody
    • BCL-5 antibody
    • BCL-6 antibody
    • BCL5 antibody
    • BCL6 antibody
    • BCL6_HUMAN antibody
    • BCL6A antibody
    • cys his2 zinc finger transcription factor antibody
    • LAZ 3 antibody
    • LAZ 3 protein antibody
    • LAZ3 antibody
    • Lymphoma associated zinc finger gene on chromosome 3 antibody
    • Protein LAZ-3 antibody
    • ZBTB 27 antibody
    • ZBTB27 antibody
    • Zinc finger and BTB domain containing protein 27 antibody
    • Zinc finger and BTB domain-containing protein 27 antibody
    • Zinc finger protein 51 antibody
    • zinc finger transcription factor BCL6S antibody
    • ZNF 51 antibody
    • ZNF51 antibody
    see all

Anti-Bcl6 antibody images

  • All lanes : Anti-Bcl6 antibody (ab86861) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
    The 100 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Bcl6.
  • All lanes : Anti-Bcl6 antibody (ab86861) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770)
    Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    The 105 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Bcl6.
  • Bcl6 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Bcl6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab86861.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to anti-rabbit HRP (ab99697).
    Band: 105kDa; Bcl6

References for Anti-Bcl6 antibody (ab86861)

This product has been referenced in:
  • Hu P  et al. Global identification of the genetic networks and cis-regulatory elements of the cold response in zebrafish. Nucleic Acids Res 43:9198-213 (2015). WB . Read more (PubMed: 26227973) »

See 1 Publication for this product

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