Overview

  • Product nameAnti-Bcl6 antibody [EP529Y]
    See all Bcl6 primary antibodies
  • Description
    Rabbit monoclonal [EP529Y] to Bcl6
  • Tested applicationsSuitable for: IP, WB, ICC, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) - corresponding to residues in the C terminal region of Bcl6.

  • Positive control
    • Daudi cell lysate, human lung adenocarcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab33901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
WB 1/500 - 1/2000. Detects a band of approximately 85-90 kDa (predicted molecular weight: 78 kDa).
ICC 1/100 - 1/250.
ICC/IF Use at an assay dependent concentration. PubMed: 19337254
IHC-P Use at an assay dependent concentration. PubMed: 19337254
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionTranscriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis.
    • Tissue specificityExpressed in germinal center T and B cells and in primary immature dendritic cells.
    • Involvement in diseaseNote=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
      Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
      Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF.
    • Sequence similaritiesContains 1 BTB (POZ) domain.
      Contains 6 C2H2-type zinc fingers.
    • DomainThe BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2.
    • Post-translational
      modifications
      Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • B cell CLL/lymphoma 6 antibody
      • B cell lymphoma 6 protein antibody
      • B-cell lymphoma 5 protein antibody
      • B-cell lymphoma 6 protein antibody
      • BCL 5 antibody
      • Bcl 6 antibody
      • BCL-5 antibody
      • BCL-6 antibody
      • BCL5 antibody
      • BCL6 antibody
      • BCL6_HUMAN antibody
      • BCL6A antibody
      • cys his2 zinc finger transcription factor antibody
      • LAZ 3 antibody
      • LAZ 3 protein antibody
      • LAZ3 antibody
      • Lymphoma associated zinc finger gene on chromosome 3 antibody
      • Protein LAZ-3 antibody
      • ZBTB 27 antibody
      • ZBTB27 antibody
      • Zinc finger and BTB domain containing protein 27 antibody
      • Zinc finger and BTB domain-containing protein 27 antibody
      • Zinc finger protein 51 antibody
      • zinc finger transcription factor BCL6S antibody
      • ZNF 51 antibody
      • ZNF51 antibody
      see all

    Anti-Bcl6 antibody [EP529Y] images

    References for Anti-Bcl6 antibody [EP529Y] (ab33901)

    This product has been referenced in:
    • Sato F  et al. Angioimmunoblastic T-cell lymphoma mice model. Leuk Res 37:21-7 (2013). Read more (PubMed: 23026775) »
    • Hirata Y  et al. BCL6 degradation caused by the interaction with the C-terminus of pro-HB-EGF induces cyclin D2 expression in gastric cancers. Br J Cancer 100:1320-9 (2009). WB, ICC/IF, IHC-P ; Human . Read more (PubMed: 19337254) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Dog Tissue sections (canine B cell lymphoma)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
    Permeabilization No
    Specification canine B cell lymphoma
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative Formaldehyde
    Username

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    Verified customer

    Submitted Dec 16 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (DLBCL cell line SUDHL6 xenograft)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
    Permeabilization No
    Specification DLBCL cell line SUDHL6 xenograft
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 15 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"