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Synthetic peptide (Human) - corresponding to residues in the C terminal region of Bcl6.
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab33901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 85-90 kDa (predicted molecular weight: 78 kDa).|
|ICC||1/100 - 1/250.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19337254|
|IHC-P||Use at an assay dependent concentration. PubMed: 19337254|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human DLBCL cell line SUDHL6 xenograft labeling Bcl6 with ab33901 at 1/500 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA pH 9. 15% serum was used to block tissue for 1 hour at 20°C. A polyclonal Goat anti-Rabbit IgG biotin conjugated secondary antibody was used at 1/200 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"