• Product nameAnti-BCL9 antibody
    See all BCL9 primary antibodies
  • Description
    Rabbit polyclonal to BCL9
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human BCL9.

    (Peptide available as ab37304.)

  • Positive control
    • This antibody gave a positive signal in the following cell lysates: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate; Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate; MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate; Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate.



Our Abpromise guarantee covers the use of ab37305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 140 kDa).Can be blocked with Human BCL9 peptide (ab37304).
IHC-P Use at an assay dependent concentration. PubMed: 22914623
ICC/IF Use a concentration of 1 µg/ml.


Anti-BCL9 antibody images

  • ICC/IF image of ab37305 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37305, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-BCL9 antibody (ab37305) at 2 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 140 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Immunohistochemical analysis of Human colorectal cancer tissue, staining BCL9 with ab37305 at 5 µg/ml.

    Left panel: BCL9 antibody alone. Right panel: BCL9 antibody + ab37304 - BCL9 peptide.

References for Anti-BCL9 antibody (ab37305)

This product has been referenced in:
  • Zhao JJ  et al. miR-30-5p functions as a tumor suppressor and novel therapeutic tool by targeting the oncogenic Wnt/ß-catenin/BCL9 pathway. Cancer Res 74:1801-13 (2014). Human . Read more (PubMed: 24599134) »
  • Hyeon J  et al. Prognostic Significance of BCL9 Expression in Hepatocellular Carcinoma. Korean J Pathol 47:130-6 (2013). IHC-P ; Human . Read more (PubMed: 23667372) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Rhesus monkey Tissue sections (Colon)
Specification Colon
Permeabilization Yes - 0.1X Triton-X-100
Fixative Z-fix

Abcam user community

Verified customer

Submitted Dec 11 2014

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (gel 12%)
Sample Human Cell lysate - whole cell (muscle)
Specification muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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Verified customer

Submitted Oct 30 2014

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We do not know what effect H2O2 stress will have on expression of BCL9 in MCF7 cells therefore I would recommend perhaps running H2O2 treated MCF7 lysate alongside untreated if possible to determine affect. Read More