![Flow Cytometry - BCMA antibody [Vicky-1] (ab17323)](http://a.abcam.com/ps/datasheet/Images/17/ab17323/ab17323_2 .jpg "Flow Cytometry - BCMA antibody [Vicky-1] (ab17323)")
- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-BCMA antibody [Vicky-1]
See all BCMA primary antibodies - DescriptionRat monoclonal [Vicky-1] to BCMA
- SpecificityRecognizes human BCMA.
- Tested applicationsSuitable for: ICC, Flow Cytmore details
- Species reactivityReacts with: Human
Does not react with: Mouse - Immunogen
Recombinant human BCMA:Fc. The extracellular domain of human BCMA (amino acids 2-54) is fused to the Fc portion of human IgG1.
- Positive control
- U266 cells
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityAffinity purified
- ClonalityMonoclonal
- Clone numberVicky-1
- IsotypeIgG1
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab17323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | ||
| Flow Cyt |
ICC: Use at a concentration of 5 µg/ml.
Functional Application (blocks binding of BAFF to human BMCA).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionReceptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
- Tissue specificityExpressed in mature B-cells, but not in T-cells or monocytes.
- Involvement in diseaseNote=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
- Sequence similaritiesContains 1 TNFR-Cys repeat.
- Cellular localizationCell membrane. Endomembrane system. Perinuclear Golgi-like structures.
- Information by UniProt
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Database links
- Entrez Gene: 608 Human
- Omim: 109545 Human
- SwissProt: Q02223 Human
- Unigene: 2556 Human
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Alternative names
- B cell maturation antigen antibody
- B cell maturation factor antibody
- B cell maturation protein antibody
see all
Anti-BCMA antibody [Vicky-1] images
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![- BCMA antibody [Vicky-1] (ab17323)](http://a.abcam.com/ps/datasheet/Images/17/ab17323/ab17323_1.jpg)
- BCMA antibody [Vicky-1] (ab17323)Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel).
Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5min at RT. Slides were blocked with normal IgG, and incubated for 1 hour with 5µ g/ml ab17323 in 1%BSA / 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. -
Flow Cytometry - BCMA antibody [Vicky-1] (ab17323)
FACS analysis of cells with ab17323 to BCMA.
Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human BCMA. Cells (5x105) were incubated on ice for 30 min in 50
µ l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µ g/ml of ab17323 monoclonal antibody to human BCMA. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. -
![Flow Cytometry / FACS - BCMA antibody [Vicky-1] (ab17323)](http://a.abcam.com/ps/datasheet/Images/17/ab17323/ab17323_3.jpg)
Flow Cytometry / FACS - BCMA antibody [Vicky-1] (ab17323)Detection of endogenous human BCMA with ab17323 to BCMA (human)(Vicky-1) (second peak, to the right). Method: U266 cells (2x105) were incubated on ice for 30 minutes with 0.2 µg of ab17323 or an isotype control in 25µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide). The primary antibody was revealed with anti- Rat IgG (R-PE) and then analyzed by Flow Cytometry.
Protocols
References for Anti-BCMA antibody [Vicky-1] (ab17323)
ab17323 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"