Overview

  • Product name
    Anti-BCNP antibody
  • Description
    Rabbit polyclonal to BCNP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human BCNP.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Bone Marrow; Human Tonsil; Human Lymph Node.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 77 kDa).

Target

Images

  • All lanes : Anti-BCNP antibody (ab113755) at 1 µg/ml

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Tonsil (Human) Tissue Lysate
    Lane 3 : Human lymph node tissue lysate - total protein (ab29871)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 241 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab113755 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (B-cell)
Loading amount
500000 cells
Specification
B-cell
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Sandrine Jayne

Verified customer

Submitted Nov 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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