Overview

  • Product nameAnti-Bcr antibody
    See all Bcr primary antibodies
  • Description
    Rabbit polyclonal to Bcr
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or WB
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Bcr aa 1250-1350 (C terminal). The exact sequence is proprietary.

  • Positive control
    • Jurkat cell lysate, human lung carcinoma and HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
IP
  • Application notesICC/IF: 1/250 - 1/500.
    IHC-P: 1/100 - 1/250.
    IP: 1/40.


    Is unsuitable for FACS or WB.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
    • Involvement in diseaseNote=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
    • Sequence similaritiesContains 1 C2 domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 Rho-GAP domain.
    • DomainThe region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
      The DH domain is involved in interaction with CCPG1.
    • Post-translational
      modifications
      Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALL antibody
      • bcr antibody
      • BCR/ABL FUSION GENE, INCLUDED antibody
      • BCR/FGFR1 chimera protein antibody
      • BCR/FGFR1 FUSION GENE, INCLUDED antibody
      • BCR/PDGFRA FUSION GENE, INCLUDED antibody
      • BCR_HUMAN antibody
      • BCR1 antibody
      • Breakpoint cluster region antibody
      • Breakpoint cluster region protein antibody
      • CML antibody
      • D22S11 antibody
      • D22S662 antibody
      • FGFR1/BCR chimera protein antibody
      • PHL antibody
      • Renal carcinoma antigen NY-REN-26 antibody
      see all

    Anti-Bcr antibody images

    • Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32523 at 1/100 dilution.Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32523 at 1/100 dilution.
    • Immunofluorescent staining of HeLa cells using ab32523 at 1/250 dilution.

    References for Anti-Bcr antibody (ab32523)

    ab32523 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab32523.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"