Overview

  • Product nameAnti-Bcr antibody
    See all Bcr primary antibodies
  • Description
    Rabbit polyclonal to Bcr
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 50 and 100 of human Bcr (NP_004318.3)

  • Positive control
    • human K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab87269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
  • Application notesICC/IF: 1/100 - 1/500.
    IHC-P: 1/100 - 1/500. Epitope exposure with citrate buffer will enhance staining.
    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
    • Involvement in diseaseNote=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
    • Sequence similaritiesContains 1 C2 domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 Rho-GAP domain.
    • DomainThe region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
      The DH domain is involved in interaction with CCPG1.
    • Post-translational
      modifications
      Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALL antibody
      • bcr antibody
      • BCR/ABL FUSION GENE, INCLUDED antibody
      • BCR/FGFR1 chimera protein antibody
      • BCR/FGFR1 FUSION GENE, INCLUDED antibody
      • BCR/PDGFRA FUSION GENE, INCLUDED antibody
      • BCR_HUMAN antibody
      • BCR1 antibody
      • Breakpoint cluster region antibody
      • Breakpoint cluster region protein antibody
      • CML antibody
      • D22S11 antibody
      • D22S662 antibody
      • FGFR1/BCR chimera protein antibody
      • PHL antibody
      • Renal carcinoma antigen NY-REN-26 antibody
      see all

    Anti-Bcr antibody images

    • ab87269 staining Bcr in Human B cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.5% Triton X-100 in PBS and blocked with 1% BSA for 90 minutes at 20°C. Samples were incubated with primary antibody (1/50; 1% BSA in PBS) for 2 hours 30 minutes at 20°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody

      See Abreview

    • ab87269 at 1/250 dilution staining Bcr in human K562 cells by Immunofluorescence, Formalin-fixed, Paraffin-embedded tissue. Detection: Red fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
    • ab87269 at 1/250 dilution staining Bcr in K562 cells by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    References for Anti-Bcr antibody (ab87269)

    ab87269 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (B lymphoma cells)
    Loading amount 100 µg
    Specification B lymphoma cells
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 22 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (B cells)
    Specification B cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 04 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"