The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: 1/100 - 1/500.
IHC-P: 1/100 - 1/500. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Involvement in diseaseNote=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
DomainThe region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene. The DH domain is involved in interaction with CCPG1.
Post-translational modificationsAutophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
Immunocytochemistry/ Immunofluorescence - Bcr antibody (ab87269)This image is courtesy of an anonymous Abreview
ab87269 staining Bcr in Human B cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.5% Triton X-100 in PBS and blocked with 1% BSA for 90 minutes at 20°C. Samples were incubated with primary antibody (1/50; 1% BSA in PBS) for 2 hours 30 minutes at 20°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody
ab87269 at 1/250 dilution staining Bcr in human K562 cells by Immunofluorescence, Formalin-fixed, Paraffin-embedded tissue. Detection: Red fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.