Overview

  • Product nameAnti-BCRP/ABCG2 antibody [BXP-21]
    See all BCRP/ABCG2 primary antibodies
  • Description
    Mouse monoclonal [BXP-21] to BCRP/ABCG2
  • SpecificityThis antibody does not react with the human MDR1, MRP1 and MRP2 gene products.
  • Tested applicationsSuitable for: ICC, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein composed of E. Coli maltose binding protein and BCRP peptide [126 amino acids, 271-396 AA of BCRP, GeneBank accession #AF098951].

  • Positive control
    • Caco Cell lysate (see second reference cited).
  • General notes

    For the WB, please note the subcellular localization and distribution of the BCRP/ABCG2 in Normal Human Tissues: PMID:11309308

    We have received mixed feedback from customers, the antibody showed 45-50 kDa band size in A549 and Caco2 cell lines against predicted size of 72kDa. In response to data we have received we no longer guarantee this antibody for western blot. ABCG2 is a multi-pass membrane protein so for troubleshooting we would recommend heating the samples at 60-70C for 15-20 minutes instead of boiling and as this protein exists as homodimer so complete reduction of samples is highly recommended. In case of any other question please email scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab3380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/20 - 1/50. Acetone fixed cells.
IHC-P 1/100.
IHC-Fr 1/20 - 1/40.
Flow Cyt Use at an assay dependent concentration. PubMed: 24136221
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionXenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
  • Tissue specificityHighly expressed in placenta. Low expression in small intestine, liver and colon.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • Post-translational
    modifications
    Glycosylation-deficient ABCG2 is normally expressed and functional.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC transporter antibody
    • ABC15 antibody
    • ABCG 2 antibody
    • ABCG2 antibody
    • ABCG2_HUMAN antibody
    • ABCP antibody
    • ATP binding cassette sub family G (WHITE) member 2 antibody
    • ATP binding cassette transporter G2 antibody
    • ATP-binding cassette sub-family G member 2 antibody
    • BCRP antibody
    • BCRP1 antibody
    • BMDP antibody
    • Breast cancer resistance protein antibody
    • CD338 antibody
    • CDw338 antibody
    • CDw338 antigen antibody
    • EST157481 antibody
    • GOUT1 antibody
    • MGC102821 antibody
    • Mitoxantrone resistance associated protein antibody
    • Mitoxantrone resistance-associated protein antibody
    • MRX antibody
    • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
    • MXR antibody
    • MXR1 antibody
    • Placenta specific ATP binding cassette transporter antibody
    • Placenta specific MDR protein antibody
    • Placenta-specific ATP-binding cassette transporter antibody
    • UAQTL1 antibody
    see all

Anti-BCRP/ABCG2 antibody [BXP-21] images

  • ab3380 staining BCRP/ABCG2 in MCF7 human breast cancer cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were then blocked using 10% serum for 2 hours at 25°C, then incubated with ab3380 at a 1/50 dilution for 12 hours at 4°C. A rabbit anti-mouse polyclonal conjugated to Alexa Fluor 594 was used as the secondary at a 1/50 dilution.

    See Abreview

  • Immunohistochemical analysis of Human breast cancer tissue, staining BCRP/ABCG2 with ab3380.

    Tissue was fixed with formaldehyde, blocked with 3% H2O2 for 5 minutes at 20°C and permeabilized with PBS-Tween; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/200 in PBS-Tween) for 1 hour at 20°C. A biotinylated goat anti-mouse polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-BCRP/ABCG2 antibody [BXP-21] (ab3380)

This product has been referenced in:
  • Curcio C  et al. The expression of LGR5 in healthy human stem cell niches and its modulation in inflamed conditions. Mol Vis 21:644-8 (2015). Read more (PubMed: 26097379) »
  • Mallery SR  et al. Clinical and biochemical studies support smokeless tobacco's carcinogenic potential in the human oral cavity. Cancer Prev Res (Phila) 7:23-32 (2014). Human . Read more (PubMed: 24265177) »

See all 30 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rabbit Tissue sections (Rabbit cornea. Limbal epithelial area. Only progen)
Specification Rabbit cornea. Limbal epithelial area. Only progen
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Dr. Noelia Andollo

Verified customer

Submitted Jun 23 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cell (ES-derived hepatocytes)
Specification ES-derived hepatocytes
Permeabilization Yes - BSA diluted in PBS/0.1% Tween
Fixative Methanol
Username

Abcam user community

Verified customer

Submitted Dec 12 2013

Thank you for your reply and these additional details.

When you write "I deparaffinized 4 times in sub-x for 5min each" I am unsure what you mean by sub-x. We recommend the following depariffinization protocol:


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Thank you for contacting us.

I am very sorry to hear of the troubles which you have been having in IHC. You have indeed matched the corrected primary and secondary antibodies for this application. Antigen retrieval using a citrate buffer s...

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Thank you for contacting us. We do not accept reviews of EMSA at this time, so I am not able to offer the testing discount for this application.

The antibody is capable of recognizing BCRP/ABCG2 in its native, 3D conformation, as suggested b...

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Thank you for contacting us. ab3380; The ABCG2 target is expressed in tonsils, spleen, gall bladder and lungs etc so the sections of these tissues will be good positive control. You can find the complete expression information on Human Protein Atlas w...

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Thank you for your enquiry. Please find enclosed the two requested CoC for ab3380 (Lot NO.:GR56451-1) and ab109374 (Lot NO.:GR49853-2) as attachments. I hope this helps and if I can assist further, please do not hesitate to contact me.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF7 human breast cancer cells)
Specification MCF7 human breast cancer cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Gerald Davies

Verified customer

Submitted Jun 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Non-reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Ms. Rhiannon Hardwick

Verified customer

Submitted Jul 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Cancer cell line)
Loading amount 15 µg
Specification Cancer cell line
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Aug 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"