Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)

Overview

  • Product nameAnti-BCRP/ABCG2 antibody [EPR2099(2)]
    See all BCRP/ABCG2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2099(2)] to BCRP/ABCG2
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the intracellular domain of Human BCRP/ABCG2.

  • Positive control
    • Human fetal liver, JAR, 293T, HepG2, and Human placenta lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionXenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
    • Tissue specificityHighly expressed in placenta. Low expression in small intestine, liver and colon.
    • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • Post-translational
      modifications
      Glycosylation-deficient ABCG2 is normally expressed and functional.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABC transporter antibody
      • ABC15 antibody
      • ABCG 2 antibody
      • ABCG2 antibody
      • ABCG2_HUMAN antibody
      • ABCP antibody
      • ATP binding cassette sub family G (WHITE) member 2 antibody
      • ATP binding cassette transporter G2 antibody
      • ATP-binding cassette sub-family G member 2 antibody
      • BCRP antibody
      • BCRP1 antibody
      • BMDP antibody
      • Breast cancer resistance protein antibody
      • CD338 antibody
      • CDw338 antibody
      • CDw338 antigen antibody
      • EST157481 antibody
      • GOUT1 antibody
      • MGC102821 antibody
      • Mitoxantrone resistance associated protein antibody
      • Mitoxantrone resistance-associated protein antibody
      • MRX antibody
      • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
      • MXR antibody
      • MXR1 antibody
      • Placenta specific ATP binding cassette transporter antibody
      • Placenta specific MDR protein antibody
      • Placenta-specific ATP-binding cassette transporter antibody
      • UAQTL1 antibody
      see all

    Anti-BCRP/ABCG2 antibody [EPR2099(2)] images

    • All lanes : Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : JAR lysate
      Lane 3 : 293T lysate
      Lane 4 : HepG2 lysate
      Lane 5 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 72 kDa

    References for Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312)

    This product has been referenced in:
    • Zhan Y  et al. Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy. Oncol Lett 12:2511-2516 (2016). Read more (PubMed: 27698820) »
    • Wu CP  et al. Identification of cancer stem-like side population cells in purified primary cultured human laryngeal squamous cell carcinoma epithelia. PLoS One 8:e65750 (2013). WB ; Human . Read more (PubMed: 23776540) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (human liver)
    Permeabilization No
    Specification human liver
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative methanol:acetone (1:1 ratio)
    Username

    Abcam user community

    Verified customer

    Submitted Oct 30 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (ES-derived hepatocytes)
    Permeabilization Yes - BSA diluted in PBS/0.1% Tween
    Specification ES-derived hepatocytes
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 80 µg
    Gel Running Conditions Reduced Denaturing (8)
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 100 µg
    Gel Running Conditions Reduced Denaturing (8)
    Sample Human Cell lysate - whole cell (HEK293T)
    Specification HEK293T
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 27 2013

    Thank you for contacting us.

    For ab108312, you could use human fetal liver (ab29890), JAR, 293T (ab95494), HepG2 (ab7900) or human placenta (ab29745) lysates as a positive control.

    For ab32574, you could consider using MRP-1 trans...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"