- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-BDH2 antibody
See all BDH2 primary antibodies -
DescriptionGoat polyclonal to BDH2
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Host speciesGoat
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide:
C-TKKKQIDQFANE
, corresponding to internal sequence amino acids 60-71 of human BDH2 (NP_064524.3). -
Positive control
- Human brain (cerebellum), kidney and liver lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab77560 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Hydrolysis
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab77560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA |
Application notes
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 27 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 27 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionDehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase.
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PathwaySiderophore biosynthesis.
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Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 515321 Cow
- Entrez Gene: 56898 Human
- Entrez Gene: 69772 Mouse
- Entrez Gene: 295458 Rat
- SwissProt: Q3T046 Cow
- SwissProt: Q9BUT1 Human
- SwissProt: Q8JZV9 Mouse
- SwissProt: D4A1J4 Rat
see all -
Alternative names
- 3 hydroxybutyrate dehydrogenase type 2 antibody
- 3-hydroxybutyrate dehydrogenase type 2 antibody
- BDH 2 antibody
see all
Images
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Western blot - BDH2 antibody (ab77560)Anti-BDH2 antibody (ab77560) at 0.3 µg/ml + human kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 1 hour
Protocols
References
ab77560 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"