Overview

  • Product name
  • Description
    Rabbit polyclonal to BDKRB1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human BDKRB1.

    (Peptide available as ab75119.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: U2OS, WERI, Saos 2

Properties

Applications

Our Abpromise guarantee covers the use of ab75120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Target

  • Function
    This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B1 bradykinin receptor antibody
    • B1BKR antibody
    • B1R antibody
    • BDKR B1 antibody
    • BDKRB 1 antibody
    • BDKRB1 antibody
    • BK 1 receptor antibody
    • BK-1 receptor antibody
    • BKB1R antibody
    • BKR 1 antibody
    • BKR1 antibody
    • BKRB1_HUMAN antibody
    • BRADY B1 antibody
    • BRADYB1 antibody
    • Bradykinin B1 receptor antibody
    • Bradykinin receptor 1 antibody
    • Bradykinin receptor B1 antibody
    see all

Images

  • All lanes : Anti-BDKRB1 antibody (ab75120) at 1 µg/ml

    Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
    Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References

ab75120 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted May 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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