Overview

  • Product nameAnti-BDNF antibody [35928.11]See all BDNF primary antibodies ...
  • Description
    Mouse monoclonal [35928.11] to BDNF
  • SpecificityMonoclonal Anti-Human BDNF will neutralize the biological activity of rhBDNF. The antibody shows < 2% cross-reactivity with rhbeta-NGF, rhNT-3 and rhNT-4 based on ELISA and immunoblotting.
  • Tested applicationsELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human BDNF (rhBDNF) expressed in Sf 21 insect cells.

  • General notesEndotoxin: <10 ng/mg by LAL method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IHC-P
  • Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml, this concentration detects 12.5 ng/well of rhBDNF.
    IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol (see Kowalski et al).
    WB: Use at a concentration of 1 - 2 µg/ml, this concentration of antibody detects BDNF at 300 ng/lane under non-reducing and reducing conditions.Predicted molecular weight: 27.8 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • Involvement in diseaseCongenital central hypoventilation syndrome
    • Sequence similaritiesBelongs to the NGF-beta family.
    • Post-translational
      modifications
      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abrineurin antibody
      • BDNF antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    References for Anti-BDNF antibody [35928.11] (ab10505)

    This product has been referenced in:
    • Hsu YY  et al. KMUP-1 attenuates serum deprivation-induced neurotoxicity in SH-SY5Y cells: roles of PKG, PI3K/Akt and Bcl-2/Bax pathways. Toxicology 268:46-54 (2010). Read more (PubMed: 19962417) »
    • Grant MM  et al. The presence of ascorbate induces expression of brain derived neurotrophic factor in SH-SY5Y neuroblastoma cells after peroxide insult, which is associated with increased survival. Proteomics 5:534-40 (2005). Read more (PubMed: 15627972) »

    See all 3 Publications for this product

    Product Wall

    Gracias por contactarnos de nuevo.

    ¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y gu...

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    Thank you for contacting us.

    I am sorry to hear you have been experiencing problems with Anti-BDNF antibody ab10505 in rat samples.

    Unfortunately, this antibody has not been tested with rat samples and therefore we cannot guarante...

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    Thank you very much for providing your protocol details. I think the 60kDa band you originally observed is a result of the homodimerization of BDNF in the samples in your first experiment so this is very promising (for example the datasheet of anoth...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"