Overview

  • Product name
    Anti-BDNF antibody [3B2]
    See all BDNF primary antibodies
  • Description
    Mouse monoclonal [3B2] to BDNF
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human BDNF (N terminal). (Purified from E.coli).
    Database link: P23560

  • Positive control
    • Brain tissue lysate, proBDNF-expressing U2OS cells; Human cerebral cortex tissue sections from Alzheimer's disease patients.
  • General notes

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

Properties

Applications

Our Abpromise guarantee covers the use of ab205067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa.
ICC/IF Use a concentration of 0.33 - 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificity
    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in disease
    Bulimia nervosa 2
    Congenital central hypoventilation syndrome
  • Sequence similarities
    Belongs to the NGF-beta family.
  • Post-translational
    modifications
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abrineurin antibody
    • ANON2 antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • BULN2 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

Images

  • All lanes : Anti-BDNF antibody [3B2] (ab205067) at 2.5 µg/ml

    Lane 1 : Human hippocampus with MES
    Lane 2 : Rat hippocampus with MES
    Lane 3 : Mouse hippocampus with MES

    Secondary
    Goat anti-Mouse800 Licor at 1/10000 dilution

    Predicted band size : 15 kDa

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel ran at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for 1 hour before incubation with ab205067 (2.5 microgram /mL) overnight at 4°C.  Antibody binding was detected using infrared labelled goat anti-mouse IgG (green) at a 1:10,000 dilution for 1 hour at room temperature.

  • Immunofluorescent analysis of U2OS cells labeling BDNF with ab205067 at 0.33 µg/ml, followed by Goat anti-mouse AlexaFluor488 secondary antibody. For nuclear staining DAPI was used. A: proBDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebral cortex tissue sections from Alzheimer's disease patients labeling BDNF with ab205067 at 5 µg/ml, followed by peroxidase secondary detection and DAB staining. A: BDNF staining by ab205067; B.Negative staining without primary antibody. Sections were counterstained with toluidine blue.

  • Anti-BDNF antibody [3B2] (ab205067) at 5 µg/ml + 10 µl of CHO cell culture supernatant containing BDNF at 10 µl

    Secondary
    HRP-conjugated Goat anti-Mouse IgG

    Predicted band size : 15 kDa

References

This product has been referenced in:
  • Li H  et al. Upregulation of miR-375 level ameliorates morphine analgesic tolerance in mouse dorsal root ganglia by inhibiting the JAK2/STAT3 pathway. J Pain Res 10:1279-1287 (2017). WB . Read more (PubMed: 28603428) »
  • Jiajie T  et al. Conserved miR-10 family represses proliferation and induces apoptosis in ovarian granulosa cells. Sci Rep 7:41304 (2017). Read more (PubMed: 28112253) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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