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Recombinant full length protein (Human)
For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.
Our Abpromise guarantee covers the use of ab24491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21667226|
|WB||1/200 - 1/2000. Predicted molecular weight: 28 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
|Inhibition Assay||1/10 - 1/50.|