This product may cross react with the following family members: NGF beta, neurotrophin 3, neurotrophin 4.
WB, IHC-P, ICC/IFmore details Unsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
Synthetic peptide within Human BDNF aa 150 to the C-terminus. The exact sequence is proprietary. Database link: P23560 (Peptide available as ab182199)
WB: U87-MG, C6, SH-SY5Y, Neuron-2a, PC-12 cell lysate, brain tissue lysate.
IHC-P: Human brain tissue.
ICC/IF: HeLa cells
Our internal data indicates that mouse and rat are not recommended for IHC.
For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Is unsuitable for Flow Cyt or IP.
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
Involvement in disease
Bulimia nervosa 2 Congenital central hypoventilation syndrome
Belongs to the NGF-beta family.
The propeptide is N-glycosylated and glycosulfated. Converted into mature BDNF by plasmin (PLG).
Western blot - Anti-BDNF antibody [EPR1292] (ab108319)
All lanes : Anti-BDNF antibody [EPR1292] (ab108319) at 1/1000 dilution
Lane 1 : Human hippocampus lysate Lane 2 : Rat hippocampus lysate Lane 3 : Mouse hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary Gt anti Rb IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa Additional bands at : 15 kDa (possible mature (processed) protein),28 kDa (possible multimer),35 kDa,45 kDa (possible immature (unprocessed)). We are unsure as to the identity of these extra bands.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108319 (1/1000) overnight at 4°C. Ab8245 (mouse anti-GAPDH; 0.05 ug/mL) was included as a loading control. Antibody binding was detected using goat anti-rabbit IgG IR-680 (green) and goat anti-mouse IgG IR800 (red) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)Image from Quirié A et al., PLoS One. 2012;7(9):e44218. doi: 10.1371/journal.pone.0044218. Epub 2012 Sep 4.; Fig 5.; September 4, 2012, PLoS ONE 7(9): e44218.
Immunohistochemical analysis of brain tissue from sedentary (SED) or exercised rats (EX), staining BDNF with ab108319.
Antigen retrieval was performed by enzymatic method at room temperature. Tissue was permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum. Samples were incubated with primary antibody (1/100) for 3 days at 3°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108319 at 1/100
Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319)This image is courtesy of an Abreview submitted by Kirk McManus (1894904)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with ab108319 at a dilution of 1/750. Cells were fixed with Paraformaldehyde and permeabilised with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.
The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.
Chen G et al. Risperidone reverses the spatial object recognition impairment and hippocampal BDNF-TrkB signalling system alterations induced by acute MK-801 treatment. Biomed Rep6:285-290 (2017).
Read more (PubMed: 28451387) »
Gideons ES et al. Chronic lithium treatment elicits its antimanic effects via BDNF-TrkB dependent synaptic downscaling. Elife6:N/A (2017).
Read more (PubMed: 28621662) »