Anti-BDP / ARID3B antibody (ab92328)
- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameAnti-BDP / ARID3B antibody
See all BDP / ARID3B primary antibodies - DescriptionRabbit polyclonal to BDP / ARID3B
- Tested applicationsSuitable for: WB, IP, IHC-Pmore details
- Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Orangutan
- Immunogen
Synthetic peptide, corresponding to a region between residues 100 and 150 of Human BDP/ ARID3B (EAW99328.1).
- Positive control
- 293T whole cell lysates
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab92328 was affinity purified using an epitope specific to BDP / ARID3B immobilized on solid support.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab92328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 61 kDa. | |
| IP | Use at 5-15 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- FunctionTranscription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A.
- Tissue specificityExpressed in placenta, testis and leukocytes. Expressed in neuroblastoma. Present in K562 erythrocytic leukemia cells (at protein level).
- Sequence similaritiesContains 1 ARID domain.
Contains 1 REKLES domain. - Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 10620 Human
- Omim: 612457 Human
- SwissProt: Q8IVW6 Human
- Unigene: 655532 Human
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Alternative names
- ARI3B_HUMAN antibody
- ARID domain containing protein 3B antibody
- ARID domain-containing protein 3B antibody
see all
Anti-BDP / ARID3B antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDP / ARID3B antibody (ab92328)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BDP / ARID3B with ab92328 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - BDP / ARID3B antibody (ab92328)All lanes : Anti-BDP / ARID3B antibody (ab92328) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Developed using the ECL technique
Predicted band size : 61 kDa
Exposure time : 3 minutes -
Immunoprecipitation - BDP / ARID3B antibody (ab92328)Detection of BDP / ARID3B by Western Blot of Immunprecipitate.
ab92328, at 1 µg/ml, staining BDP / ARID3B in 293T whole cell lysate immunoprecipitated using ab92328 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-BDP / ARID3B antibody (ab92328)
This product has been referenced in:
- Wood JJ et al. ARID3B: a Novel Regulator of the Kaposi's Sarcoma-Associated Herpesvirus Lytic Cycle. J Virol 90:9543-55 (2016). Read more (PubMed: 27512077) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"