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Rabbit polyclonal to BDP / ARID3B
Predicted to work with:
Rhesus monkey, Orangutan
Synthetic peptide, corresponding to a region between residues 100 and 150 of Human BDP/ ARID3B (EAW99328.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab92328 was affinity purified using an epitope specific to BDP / ARID3B immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
Use at 5-15 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Transcription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A.
Expressed in placenta, testis and leukocytes. Expressed in neuroblastoma. Present in K562 erythrocytic leukemia cells (at protein level).
Contains 1 ARID domain.
Contains 1 REKLES domain.
Information by UniProt
ARID domain containing protein 3B antibody
ARID domain-containing protein 3B antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDP / ARID3B antibody (ab92328)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BDP / ARID3B with ab92328 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - BDP / ARID3B antibody (ab92328)
All lanes : Anti-BDP / ARID3B antibody (ab92328) at 0.1 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 5 µg Developed using the ECL technique Predicted band size : 61 kDa Exposure time : 3 minutes
Immunoprecipitation - BDP / ARID3B antibody (ab92328)
Detection of BDP / ARID3B by Western Blot of Immunprecipitate.
ab92328, at 1 µg/ml, staining BDP / ARID3B in 293T whole cell lysate immunoprecipitated using ab92328 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
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