Overview

  • Product name
    Anti-Beclin 1 antibody [2A4]
    See all Beclin 1 primary antibodies
  • Description
    Mouse monoclonal [2A4] to Beclin 1
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of human Beclin 1 expressed in E. Coli.

  • Positive control
    • WB: Hela; A431; MCF7; RAJI; Jurkat and SKBR3 cell lysates IHC-P: Breast cancer and liver cancer tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab114071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 60 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the beclin family.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Anti-Beclin 1 antibody [2A4] images

  • All lanes : Anti-Beclin 1 antibody [2A4] (ab114071) at 1/500 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Raji cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : SKBR3 cell lysate


    Predicted band size : 60 kDa
  • Immunohistochemical analysis of Beclin 1 expression in paraffin embedded breast cancer tissues (left) and liver cancer tissues (right) using ab114071 at 1/200 dilution with DAB staining
  • Flow cytometric analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).

References for Anti-Beclin 1 antibody [2A4] (ab114071)

This product has been referenced in:
  • Liu H  et al. PI3K/AKT/mTOR pathway promotes progestin resistance in endometrial cancer cells by inhibition of autophagy. Onco Targets Ther 10:2865-2871 (2017). WB ; Human . Read more (PubMed: 28652768) »
  • Jiang W  et al. SIRT1 protects against apoptosis by promoting autophagy in degenerative human disc nucleus pulposus cells. Sci Rep 4:7456 (2014). Human . Read more (PubMed: 25503852) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (Canine osteosarcoma & mammary tumour)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Treatment
1-2 (OS1) = N/A, 3 (D17) = 75 uM HCQ, 4 (D17) = 75 uM HCQ + doxorubicin, 5 (D17) = 75 uM HCQ + 5 uM Spautin-1, 6 (D17) = 75 uM HCQ + 5 uM Spautin-1 + doxorubicin, 7 (CF41) = N/A, 8 (MCF7) = N/A, 9 (D17) = N/A, 10 (D17) = doxorubicin, 11 (D17) = 15 uM Spautin-1 + doxorubicin, 12 (D17) = 120 uM Spautin-1 + doxorubicin, 13 (SKBR3) = N/A, 14 (OS2) = E64d + pepstatin-A (All treatments 6 hr except lane 14 which was 24 hr)
Specification
Canine osteosarcoma & mammary tumour
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Courtney Schott

Verified customer

Submitted Sep 26 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triton x 100: 30 min
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 05 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 02 2016

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12)
Loading amount
30 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Md. Golam Sharoar

Verified customer

Submitted Feb 16 2016

Thank you for contacting us.

Your credit note ID is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask...

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Thank you for contacting us.

My colleague John has forwarded you the Flow Cytometry protocol used by our lab, please let us know if you have any further questions about this.

Unfortunately, the concentration for this antibody was no...

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Thank you for contacting Abcam yesterday.

Please find attached the protocol for Flow Cytometry that was used to test ab114071. The secondary antibody used was a AlexaFluor@488goatanti-mouse(IgG(H+C), dilution:1:150.
Please let me know if...

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Code de remise : ********
Date d’expiration : JJ MM AAAA
Je suis ravi que vous souhaitiez participer à notre offre et tester ab114071 en ICC/IF. Le code de réduction correspondant est ******. Ce code de réduction est utilisable sous 120 jours...

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I apologize, I had sent the wrong link.  Here are the two references for ab114071: http://www.abcam.com/Beclin-1-antibody-2A4-ab114071-references.html

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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