Overview

  • Product nameAnti-Beclin 1 antibodySee all Beclin 1 primary antibodies ...
  • Description
    Rabbit polyclonal to Beclin 1
  • Tested applicationsICC/IF, WB, IP, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Human) (Internal sequence within residues 1-100).

Properties

Applications

Our Abpromise guarantee covers the use of ab16998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/50 - 1/200.
WB Use at an assay dependent dilution. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
1/100 (lot A), 1/2000-1/5000 (current lot).

This datasheet has changed from the Lot A datasheet (sold out). Please note the concentration and suggested dilution changes.
IP 1/80. (lot A).
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionPlays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the beclin family.
  • Cellular localizationGolgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Target information above from: UniProt accession Q14457 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • BECN1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Anti-Beclin 1 antibody images

  • Anti-Beclin 1 antibody (ab16998) + Mouse liver tissue lysate at 50 µg

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ICC/IF image of ab16998 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16998, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Beclin 1 antibody (ab16998)

This product has been referenced in:
  • Nuñez CE  et al. Defective regulation of adipose tissue autophagy in obesity. Int J Obes (Lond) N/A:N/A (2013). Read more (PubMed: 23478428) »
  • Herberg S  et al. Stromal cell-derived factor-1ß mediates cell survival through enhancing autophagy in bone marrow-derived mesenchymal stem cells. PLoS One 8:e58207 (2013). WB ; Mouse . Read more (PubMed: 23472159) »

See all 6 Publications for this product

Product Wall

Thank you for your enquiry. It seems like you are interested in using ab16998 in IHC on paraffin embeded sections but visualizing the staining via fluorescence. If this is the case you can certainly use ab16998 in your proposed experiment. I would also...

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Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 40 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2011

Application Western blot
Sample Mouse Tissue lysate - whole (heart tissue)
Loading amount 50 µg
Specification heart tissue
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 17 2010

Thank you for your enquiry. Unfortunately the immunogens for ab16998 and ab37007 are proprietary information. These are sourced antibodies and I do not have this information to hand. I can however tell you that ab22742 forms a poor sequence alignme...

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Thank you for your enquiry. The only Beclin 1 antibody that we currently carry is ab16998, there are not any alternatives. Please contact us again if you have any additional questions.

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Hela)
Specification Hela
Immuno-precipitation step Protein A
Username

Dr. Stephen Dann

Verified customer

Submitted Dec 14 2005

Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Specification Hela
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Dr. Stephen Dann

Verified customer

Submitted Dec 14 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"