The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Sequence similaritiesBelongs to the beclin family.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
Beclin 1 autophagy related antibody
BECN 1 antibody
Coiled coil myosin like BCL2 interacting protein antibody
Coiled-coil myosin-like BCL2-interacting protein antibody
Protein GT197 antibody
VPS 30 antibody
Anti-Beclin 1 antibody images
Western blot - Beclin 1 antibody (ab92389)
All lanes : Anti-Beclin 1 antibody (ab92389)
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 52 kDa
Exposure time : 3 minutes
Immunoprecipitation - Beclin 1 antibody (ab92389)
Sample: HeLa whole cell lysate (1mg for IP, 20% of IP loaded).
Antibodies: ab92389 used for WB 1µg/ml (lane 1)
and used for IP at 3µg/mg lysate. Lane 2: control IgG.
Detection: chemiluminescence with exposure time of 10 seconds.
References for Anti-Beclin 1 antibody (ab92389)
has not yet been referenced specifically in any publications.
Publishing research using ab92389? Please let us know so that we can cite the reference in this datasheet.
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