The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Sequence similaritiesBelongs to the beclin family.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
Beclin 1 autophagy related antibody
BECN 1 antibody
Coiled coil myosin like BCL2 interacting protein antibody
Coiled-coil myosin-like BCL2-interacting protein antibody
Protein GT197 antibody
VPS 30 antibody
Anti-Beclin 1 antibody images
Western blot - Beclin 1 antibody (ab92389)
All lanes : Anti-Beclin 1 antibody (ab92389)
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 52 kDa
Exposure time : 3 minutes
Immunoprecipitation - Beclin 1 antibody (ab92389)
Sample: HeLa whole cell lysate (1mg for IP, 20% of IP loaded).
Antibodies: ab92389 used for WB 1µg/ml (lane 1)
and used for IP at 3µg/mg lysate. Lane 2: control IgG.
Detection: chemiluminescence with exposure time of 10 seconds.
References for Anti-Beclin 1 antibody (ab92389)
has not yet been referenced specifically in any publications.
Publishing research using ab92389? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"