Purification notesWhole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
Use 1µl for 102 cells.
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionPlays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Sequence similaritiesBelongs to the beclin family.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.