Overview

  • Product nameAnti-BEST4 antibody
    See all BEST4 primary antibodies
  • Description
    Rabbit polyclonal to BEST4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: TVSYTLKVAE ARFGGFSGLL LRWRGSIYKL LYKEFLLFGA LYAVLSITYR , corresponding to amino acids 2-51 of Human BEST4

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceBEST4 (Bestrophin 4) is a member of the bestrophin gene family of anion channels. Bestrophins are transmembrane spanning proteins that share a homology region with a high content of aromatic residues including an invariant arginine (R), phenylalanine (F), and proline (P) motif. BEST4 may form calcium-sensitive chloride channels and may conduct other physiologically significant anions such as bicarbonate.
  • Cellular localizationCell membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • BEST 4 antibody
    • Bestrophin 4 antibody
    • Bestrophin4 antibody
    • MGC126872 antibody
    • Vitelliform macular dystrophy 2 like 2 antibody
    • Vitelliform macular dystrophy 2 like protein 2 antibody
    • VMD2-like gene 2 antibody
    • VMD2L2 antibody
    see all

Anti-BEST4 antibody images

  • Anti-BEST4 antibody (ab66632) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-BEST4 antibody (ab66632)

ab66632 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (Brain)
Loading amount 30 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (Invitrogen 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Nov 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"