Anti-Bestrophin 3 antibody (ab94904)

Overview

  • Product nameAnti-Bestrophin 3 antibody
  • Description
    Rabbit polyclonal to Bestrophin 3
  • SpecificityThe immunogen shows 100% homology with Bestrophin 3 isoforms 1 and 2. The immunogen also shows 76% homology with bestrophin 1 and 68% bestrophin 2. We predict it will react based on similar homology, but this cross-reactivity has not been tested.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (LVYTQVAEQL INPFGEDDDD FETNWCIDRN LQVSLLAVDE MHMSLPKMKK) of Human Bestrophin 3 (NP_689652).

  • Positive control
    • Human fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionForms calcium-sensitive (By similarity) chloride channels. Permeable to bicarbonate.
  • Tissue specificityPresent in skeletal muscle and weakly in brain, spinal cord, bone marrow and retina.
  • Sequence similaritiesBelongs to the bestrophin family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BEST 3 antibody
    • BEST3 antibody
    • BEST3_HUMAN antibody
    • Bestrophin-3 antibody
    • Bestrophin3 antibody
    • MGC13168 antibody
    • MGC40411 antibody
    • OTTHUMP00000195114 antibody
    • OTTHUMP00000233491 antibody
    • Vitelliform macular dystrophy 2 like 3 antibody
    • Vitelliform macular dystrophy 2 like protein 3 antibody
    • Vitelliform macular dystrophy 2-like protein 3 antibody
    • VMD2L3 antibody
    see all

Anti-Bestrophin 3 antibody images

  • Anti-Bestrophin 3 antibody (ab94904) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
  • Anti-Bestrophin 3 antibody (ab94904) at 1/500 dilution (in PBS-T (0.1%) for 18 hours at 4°C) + Mouse heart whole tissue lysate at 30 µg

    Secondary
    A Biotin-conjugated Goat anti-rabbit IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 76.4 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Bestrophin 3 antibody (ab94904)

ab94904 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (heart)
Loading amount 30 µg
Specification heart
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
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Submitted Sep 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"