Overview

  • Product nameAnti-Bestrophin antibody
    See all Bestrophin primary antibodies
  • Description
    Rabbit polyclonal to Bestrophin
  • Tested applicationsSuitable for: IP, WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    LLYGEFLIFLLCYYIIRFIYRL

    , corresponding to amino acids 31-52 of Human Bestrophin. The peptide was amidated for conjugation.

  • Positive control
    • Conditioned medium cultured RPE cells (the basal expression of Bestrophin is low in cultured cells).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: Tris glycine, 0.5% BSA, 30% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
ICC
ICC/IF
  • Application notesICC: Use at an assay dependent dilution.
    IP: 1/200.
    WB: 1/500. Predicted molecular weight: 68 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionForms calcium-sensitive chloride channels. Highly permeable to bicarbonate.
    • Tissue specificityPredominantly expressed in the basolateral membrane of the retinal pigment epithelium.
    • Involvement in diseaseDefects in BEST1 are the cause of vitelliform macular dystrophy type 2 (VMD2) [MIM:153700]; also known as Best macular dystrophy (BMD). VMD2 is an autosomal dominant form of macular degeneration that usually begins in childhood or adolescence. VMD2 is characterized by typical 'egg-yolk' macular lesions due to abnormal accumulation of lipofuscin within and beneath the retinal pigment epithelium cells. Progression of the disease leads to destruction of the retinal pigment epithelium and vision loss.
      Defects in BEST1 are the cause of retinitis pigmentosa type 50 (RP50) [MIM:613194]. A retinal dystrophy belonging to the group of pigmentary retinopathies. RP is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
      Defects in BEST1 are a cause of adult-onset vitelliform macular dystrophy (AVMD) [MIM:608161]. AVMD is a rare autosomal dominant disorder with incomplete penetrance and highly variable expression. Patients usually become symptomatic in the fourth or fifth decade of life with a protracted disease of decreased visual acuity.
      Defects in BEST1 are the cause of bestrophinopathy autosomal recessive (ARB) [MIM:611809]. A retinopathy characterized by central visual loss, an absent electro-oculogram light rise, and a reduced electroretinogram.
      Defects in BEST1 are the cause of vitreoretinochoroidopathy autosomal dominant (ADVIRC) [MIM:193220]. A disorder characterized by vitreoretinochoroidal dystrophy. The clinical presentation is variable and may be associated with cataract, nanophthalmos, microcornea, shallow anterior chamber, and glaucoma.
    • Sequence similaritiesBelongs to the bestrophin family.
    • Post-translational
      modifications
      Phosphorylated by PP2A.
    • Cellular localizationCell membrane. Basolateral cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARB antibody
      • BEST 1 antibody
      • BEST antibody
      • Best disease antibody
      • Best macular dystrophy antibody
      • BEST1 antibody
      • BEST1_HUMAN antibody
      • Best1V1Delta2 antibody
      • Bestrophin 1 antibody
      • Bestrophin-1 antibody
      • Bestrophin1 antibody
      • BMD antibody
      • mBest1 antibody
      • RP50 antibody
      • TU15B antibody
      • Vitelliform macular dystrophy 2 antibody
      • Vitelliform macular dystrophy antibody
      • Vitelliform macular dystrophy protein 2 antibody
      • VMD 2 antibody
      • VMD2 antibody
      see all

    Anti-Bestrophin antibody images

    • ab14927 staining HEK-293 cells transfected with human Bestrophin 1 by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% goat serum for 20 minutes at 25°C.  The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.  Bestrophin transfected in HEK-293 cells is labeled with green fluorescence. Nuclei were counterstained with DAPI (blue).

      See Abreview

    • All lanes : Anti-Bestrophin antibody (ab14927) at 1/200 dilution

      Lane 1 : Whole cell lysate from bovine retinal pigment epithelial cells
      Lane 2 : Whole cell lysate from non transfected HEK 293 cells

      Lysates/proteins at 25 µg per lane.

      Secondary
      HRP conjugated goat anti-rabbit at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 68 kDa
      Observed band size : 68 kDa
      Additional bands at : 74 kDa (possible non-specific binding).

      Exposure time : 20 minutes

      This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

      See Abreview

    References for Anti-Bestrophin antibody (ab14927)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Thank you for your response.

    It is important to clarify that the discount code will be activated after submission of the Abreview and there is normally an expiry date (4 month after offering the code) for sending the Abreview.

    I wou...

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    Thank you very much for your interest in ab14927.

    To our knowledge,ab14927 has not been tested in Cell sorting/FACS. Therefore, I can offer a discount off a future purchase if you buyab14927 now, test it inCell sorting/FACS and submit feedba...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Cow Cell lysate - whole cell (RPE, bovine retinal pigment epithelial cells)
    Loading amount 25 µg
    Specification RPE, bovine retinal pigment epithelial cells
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Feb 22 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HEK-293 cells transfected with human Bestrophin 1)
    Specification HEK-293 cells transfected with human Bestrophin 1
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% TX 100, 5% goat serum, 1XPBS
    Blocking step goat serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Feb 15 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HEK-293 cells transfected with hBestrophin 1)
    Total protein in input 20 µg
    Specification HEK-293 cells transfected with hBestrophin 1
    Immuno-precipitation step Protein G
    Username

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Feb 15 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK 293 transfected with human bestrophin 1)
    Loading amount 20 µg
    Specification HEK 293 transfected with human bestrophin 1
    Gel Running Conditions Reduced Denaturing (10 %)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Feb 15 2008

    Thank you for your email. At this time, the blocking peptide is not available for sale. Without knowing additional details regarding your protocol, I would like to make the following suggestions in order to try to help you out. Decrease the amo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"