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Our Abpromise guarantee covers the use of ab2182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab2182 staining pig retinal pigment epithelium tissue sections. The tissue was fixed with paraformaldehyde, permeabilised in 0.5% TX100, 5% goat serum, 1XPBS, and blocked with 5% serum for 20 minutes at 25°C. The primary anitbody was diluted 1/500 in 1% goat serum, 0.1% TX100, 1XPBS and incubated for 12 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-mouse was used as the secondary antibody.
The image shows bestrophin, which localises to the retinal pigment epithelial (RPE) cells, labeled with green fluorescence. Nuclei were counterstained with DAPI (blue).
This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
ab2182 staining cells of bovine retinal pigment epithelium (RPE) by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% goat serum for 20 minutes at 25°C. The primary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-mouse antibody was used as the secondary. Image A shows bestrophin localized in the basolateral side of the RPE cells labeled with red fluorescence (nuclei were counterstained with DAPI (blue)). Image B, a phase contrast (DIC) image, shows cellular structure.
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