Anti-beta 1 Adrenergic Receptor antibody (ab3442)

Overview

  • Product nameAnti-beta 1 Adrenergic Receptor antibody
    See all beta 1 Adrenergic Receptor primary antibodies
  • Description
    Rabbit polyclonal to beta 1 Adrenergic Receptor
  • Tested applicationsWB, ICC/IF, IP, IHC-FoFr, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Human, Non Human Primates, African Green Monkey, Prairie Vole
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse beta 1 Adrenergic Receptor aa 394-408.
    Sequence:

    HGDRPRASGCLARAG

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notes Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.
  • Clonality Polyclonal
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab3442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50.5 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/100. Immunofluorescence staining of B1AR in mouse kidney distal tubule yields a pattern consistent with plasma membrane staining.
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
ICC 1/5000.
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
  • DomainThe PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
  • Post-translational
    modifications
    Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
  • Cellular localizationCell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADR B1 antibody
    • ADRB 1 antibody
    • ADRB1 antibody
    • ADRB1_HUMAN antibody
    • ADRB1R antibody
    • Adrenergic beta 1 receptor antibody
    • Adrenoceptor beta 1 antibody
    • B1AR antibody
    • Beta 1 adrenoceptor antibody
    • Beta 1 adrenoreceptor antibody
    • Beta-1 adrenergic receptor antibody
    • Beta-1 adrenoceptor antibody
    • Beta-1 adrenoreceptor antibody
    • BETA1AR antibody
    • RHR antibody
    see all

Anti-beta 1 Adrenergic Receptor antibody images

  • All lanes : Anti-beta 1 Adrenergic Receptor antibody (ab3442) at 1/1000 dilution

    Lane 1 : As above
    Lane 2 : As above
    Lane 3 : As above
    Lane 4 : As above
    Lane 5 : As above
    Lane 6 : As above
    Lane 7 : As above
    Lane 8 : As above
    Lane 9 : As above
    Lane 10 : As above
    Lane 11 : As above
    Lane 12 : As above

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit at 1/20000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
    Western blot analysis of ADR-Beta-1 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with ab3442 at a dilution of 1/1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1/20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.
  • ab3442 at 1/100 dilution staining beta 1 Adrenergic Receptor in untreated HeLa cells by Immunocytochemistry/ Immunofluorescence. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1, at a dilution of 1/100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488-conjugated goat-anti-rabbit secondary antibody at a dilution of 1/400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye.
  • ab3442 staining the beta 1 Adrenergic Receptor in mouse bone marrow cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.5% BSA in 2mM EDTA/PBS. The sample was incubated with the primary antibody (1/100) for 20 minutes at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
    Gating Strategy: Blood cells.

    See Abreview

References for Anti-beta 1 Adrenergic Receptor antibody (ab3442)

This product has been referenced in:
  • Tang C  et al. Phosphorylation of beta-adrenergic receptor leads to its redistribution in rat heart during sepsis. Am J Physiol 274:R1078-86 (1998). Read more (PubMed: 9575972) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0.1%spaonin/PBS
Sample Mouse Cell (bone marrow)
Specification bone marrow
Gating Strategy blood cells
Preparation Cell harvesting/tissue preparation method: obtained from murine femur frushed with RPMI
Sample buffer: 0.5% BSA/2mM EDTA/PBS
Username

Abcam user community

Verified customer

Submitted Jun 23 2014

Thank you for bringing this to our attention. The two bands you are seeing might represent unphosphorylated (lower band) and fully phosphorylated (upper band) receptor but that is unlikely if the difference in sizes is 5 kDa and you are seeing just two...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Prairie Vole Cell (brain)
Specification brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2012

Thank you very much for your inquiry. I apologize for the long delay of my answer.

Firstly, thank you very much for having provided such a nice summary, including the data and the protocol steps. This makes it easier for us, to understand the...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital informati...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Thank you very much for your interest in our adrenergic Receptor antibodies. These are the alternatives we could recommend: Anti-beta 1 Adrenergic Receptor antibody (replacement for ab3546): The rabbit polyclonal ab3442 which is guaranteed to wo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"