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Western blot - beta 1 Adrenergic Receptor antibody (ab64996)
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Product Name
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beta 1 Adrenergic Receptor antibody
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See all beta 1 Adrenergic Receptor antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to beta 1 Adrenergic Receptor
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Immunogen
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Synthetic peptide derived from internal sequence of human beta 1 Adrenergic Receptor.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HT-29 cells.
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Cellular localization
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Cell membrane; Multi-pass membrane protein. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
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Research areas
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Cardiovascular >> Blood >> Blood Pressure regulation
Signal Transduction >> Growth Factors/Hormones >> Adrenalin
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Adrenergic Receptors
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Relevance
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ADRB1 is an beta-adrenergic receptor. Beta receptors are coupled to Gs proteins, which in turn are linked to adenylyl cyclase. Agonist binding thus causes a rise in the intracellular concentration of the second messenger cAMP.
Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence.
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beta 1 Adrenergic Receptor antibody (ab64996) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta 1 Adrenergic Receptor antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for beta 1 Adrenergic Receptor antibody images, references, products related to ab64996 and other tools.
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beta 1 Adrenergic Receptor antibody images:
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 Western blot - beta 1 Adrenergic Receptor antibody (ab64996)
All lanes : beta 1 Adrenergic Receptor antibody (ab64996) at 1/500 dilution
Lane 1 : Extracts from HT-29 cells (5-30 µg of total protein)
Lane 2 : Extracts from HT-29 cells (5-30 µg of total protein) with 5-10µg of the immunising peptide
Predicted band size : 51 kDa
Observed band size : 51 kDa
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Search PubMed (MEDLINE) for references to beta 1 Adrenergic Receptor
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beta 1 Adrenergic Receptor antibody - more information
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Customer reviews (feedback) regarding beta 1 Adrenergic Receptor antibody |
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beta 1 Adrenergic Receptor antibody - related products:
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beta 1 Adrenergic Receptor antibody - other tools:
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