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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Adrenergic Receptors
Overview
Product name
Anti-beta 1 Adrenergic Receptor antibody
See all beta 1 Adrenergic Receptor products (5) ...
Description
Goat polyclonal to beta 1 Adrenergic Receptor
Tested applications
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Guinea pig, Cat, Dog, Rhesus monkey, Gerbil
Immunogen
Synthetic peptide: ESDEARRCYNDPK from the internal region of Human beta 1 Adrenergic Receptor
ESDEARRCYN DPK
Positive control
Human heart lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Blood Pressure regulation
Signal Transduction >> Growth Factors/Hormones >> Adrenalin
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Adrenergic Receptors
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Western blot - beta 1 Adrenergic Receptor antibody (ab77189)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml.Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
Target
Function
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
Domain
The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
Post-translational
modifications
Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
Cellular localization
Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
Target information above from: UniProt accessionP08588
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADR B1 antibody
- ADRB 1 antibody
- ADRB1 antibody
see all
Database links
- Entrez Gene: 493971 Cat
- Entrez Gene: 493972 Dog
- Entrez Gene: 153 Human
- Entrez Gene: 11554 Mouse
- Entrez Gene: 24925 Rat
- Omim: 109630 Human
- SwissProt: Q9TST6 Cat
- SwissProt: P79148 Dog
see all
Anti-beta 1 Adrenergic Receptor antibody images:
Western blot - beta 1 Adrenergic Receptor antibody (ab77189)
Anti-beta 1 Adrenergic Receptor antibody (ab77189) at 0.3 µg/ml + Human heart lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 51 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 hour
References for Anti-beta 1 Adrenergic Receptor antibody (ab77189)
This product has been referenced in:
- Wallukat Get al. Distinct patterns of autoantibodies against G-protein-coupled receptors in Chagas' cardiomyopathy and megacolon. Their potential impact for early risk assessment in asymptomatic Chagas' patients. J Am Coll Cardiol 55:463-8 (2010). Rat.Read more (PubMed: 20117461) »
See 1 publication for this product
Publishing research using ab77189? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"