Anti-beta 1 Adrenergic Receptor antibody (ab77189)

Overview

  • Product nameAnti-beta 1 Adrenergic Receptor antibody
    See all beta 1 Adrenergic Receptor primary antibodies
  • Description
    Goat polyclonal to beta 1 Adrenergic Receptor
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human
    Predicted to work with: Mouse, Guinea pig, Cat, Rhesus monkey, Gerbil
  • Immunogen

    Synthetic peptide:

    ESDEARRCYNDPK

    from the internal region of Human beta 1 Adrenergic Receptor

  • Positive control
    • Human heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
  • DomainThe PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
  • Post-translational
    modifications
    Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
  • Cellular localizationCell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADR B1 antibody
    • ADRB 1 antibody
    • ADRB1 antibody
    • ADRB1_HUMAN antibody
    • ADRB1R antibody
    • Adrenergic beta 1 receptor antibody
    • Adrenoceptor beta 1 antibody
    • B1AR antibody
    • Beta 1 adrenoceptor antibody
    • Beta 1 adrenoreceptor antibody
    • Beta-1 adrenergic receptor antibody
    • Beta-1 adrenoceptor antibody
    • Beta-1 adrenoreceptor antibody
    • BETA1AR antibody
    • RHR antibody
    see all

Anti-beta 1 Adrenergic Receptor antibody images

  • Anti-beta 1 Adrenergic Receptor antibody (ab77189) at 0.3 µg/ml + Human heart lysate in RIPA buffer at 35 µg
    developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-beta 1 Adrenergic Receptor antibody (ab77189)

This product has been referenced in:
  • Schuller HM  et al. Regulation of pancreatic cancer by neuropsychological stress responses: a novel target for intervention. Carcinogenesis 33:191-6 (2012). WB ; Human . Read more (PubMed: 22072614) »
  • Wallukat G  et al. Distinct patterns of autoantibodies against G-protein-coupled receptors in Chagas' cardiomyopathy and megacolon. Their potential impact for early risk assessment in asymptomatic Chagas' patients. J Am Coll Cardiol 55:463-8 (2010). Rat . Read more (PubMed: 20117461) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-15%)
Sample Dog Tissue lysate - whole (heart)
Specification heart
Blocking step (agent) for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 14 2014


I am very pleased to hear you would like to accept our offer and testab77189 inwestern blot/flow.This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forwestern blotand includ...

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Thank you for confirming the order number.

ab77189, one vial has been added to your next order 1151187.

Thanks you very much for your cooperation.

I am sorry I can't send you the antibody without receiving the purchase order number. You need to contact our distributor Kimera for this. They can contact us on your behalf with purchase order number. So please cotnact them and discuss.

Thanks!

Could you provide the purchase order number for us to send the requested replacement antibodies?

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Thank you for getting back to me.

I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the ap...

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Thank you for providing some information.

Could you please specify what size are the non-specific bands?

Are they lower or higher than the expected size (51 kDa)?

Could you attach an image (saved as a jpeg file) to your e-...

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Thank you for your recent e-mail and for sending the Western blot image to our Scientific Support Team.

My colleague is out of office for two weeks and he has asked me to look after her customers during her absence.

Could you provid...

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I am sorry to hear that you are experiencing problems again.

Could you summarize the results? Do you observe high back ground or no staining. I presuming you are not observing band of interest at different size so could you provide image of st...

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Thank you for contacting us.

You can choose any, both will work for 4 hours incubations. However with overnight incubation 4C is recommended.

Please let me know how the antibody performs.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"