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Our Abpromise guarantee covers the use of ab3442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 50.5 kDa (predicted molecular weight: 50 kDa).|
|ICC/IF||1/100. Immunofluorescence staining of B1AR in mouse kidney distal tubule yields a pattern consistent with plasma membrane staining.|
|IP||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ab3442 staining the beta 1 Adrenergic Receptor in mouse bone marrow cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.5% BSA in 2mM EDTA/PBS. The sample was incubated with the primary antibody (1/100) for 20 minutes at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Gating Strategy: Blood cells.
Immunolocalization of B1AR in mouse kidney distal tubule using ab3442.