Anti-beta 1 Sodium Potassium ATPase antibody (ab96292)

Overview

  • Product nameAnti-beta 1 Sodium Potassium ATPase antibody
    See all beta 1 Sodium Potassium ATPase primary antibodies
  • Description
    Rabbit polyclonal to beta 1 Sodium Potassium ATPase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Guinea pig, Dog, Pig
  • Immunogen

    Recombinant fragment corresponding to Human beta 1 Sodium Potassium ATPase aa 83-303.
    Database link: P05026

  • Positive control
    • MOLT4, A431, HepG2 and Raji cell lysates for WB and MDAMB468 xenograft for IHC-P

Properties

Applications

Our Abpromise guarantee covers the use of ab96292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.

Optimal dilutions/concentrations should be determined by the researcher

IHC-P 1/100 - 1/1000.

Optimal dilutions/concentrations should be determined by the researcher

Target

  • FunctionThis is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
  • Tissue specificityFound in most tissues.
  • Sequence similaritiesBelongs to the X(+)/potassium ATPases subunit beta family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosinetriphosphatase antibody
    • AT1B1_HUMAN antibody
    • ATP 1B antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • Atpb 1 antibody
    • ATPBS antibody
    • Beta 1 subunit of Na(+) K(+) ATPase antibody
    • Na K ATPase beta 1 polypeptide antibody
    • Na+/K+ ATPase beta 1 subunit antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • Sodium/potassium-dependent ATPase subunit beta-1 antibody
    • Sodium/potassium-transporting ATPase subunit beta-1 antibody
    see all

Anti-beta 1 Sodium Potassium ATPase antibody images

  • ab96292 detects ATP1B1 protein at membrane on hepatoma by immunohistochemcial analysis. Sample: Paraffin-embedded hepatoma tissue. Antibody dilution: 1:500

  • ab96292 detects ATP1B1 protein at membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Antibody dilution: 1:500

  • Anti-beta 1 Sodium Potassium ATPase antibody (ab96292) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 35 kDa
    10% SDS-PAGE
  • ab96292, at 1/100 dilution, staining beta Sodium Potassium ATPase in paraffin-embedded MDAMB468 xenograft by Immunohistochemistry.

References for Anti-beta 1 Sodium Potassium ATPase antibody (ab96292)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (MDCK)
Loading amount 15 µg
Specification MDCK
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"