Recombinant
RabMAb

Anti-beta 1 Sodium Potassium ATPase antibody [EPR12195] (ab193669)

Overview

  • Product name
    Anti-beta 1 Sodium Potassium ATPase antibody [EPR12195]
    See all beta 1 Sodium Potassium ATPase primary antibodies
  • Description
    Rabbit monoclonal [EPR12195] to beta 1 Sodium Potassium ATPase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human beta 1 Sodium Potassium ATPase aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P05026

  • Positive control
    • Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain and Rat heart lysates; Human renal adenocarcinoma tissue; Mouse liver tissue; Rat kidney tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab193669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
  • Tissue specificity
    Found in most tissues.
  • Sequence similarities
    Belongs to the X(+)/potassium ATPases subunit beta family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosinetriphosphatase antibody
    • AT1B1_HUMAN antibody
    • ATP 1B antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • Atpb 1 antibody
    • ATPBS antibody
    • Beta 1 subunit of Na(+) K(+) ATPase antibody
    • Na K ATPase beta 1 polypeptide antibody
    • Na+/K+ ATPase beta 1 subunit antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • Sodium/potassium-dependent ATPase subunit beta-1 antibody
    • Sodium/potassium-transporting ATPase subunit beta-1 antibody
    see all

Images

  • Anti-beta 1 Sodium Potassium ATPase antibody [EPR12195] (ab193669) at 1/10000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 45-50 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-beta 1 Sodium Potassium ATPase antibody [EPR12195] (ab193669) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 45-50 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-beta 1 Sodium Potassium ATPase antibody [EPR12195] (ab193669) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 45-50 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling beta 1 Sodium Potassium ATPase with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling beta 1 Sodium Potassium ATPase with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling beta 1 Sodium Potassium ATPase with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

References

ab193669 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193669.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up