Anti-beta 1 Spectrin antibody [DB2] (ab86185)

Overview

  • Product nameAnti-beta 1 Spectrin antibody [DB2]
    See all beta 1 Spectrin primary antibodies
  • Description
    Mouse monoclonal [DB2] to beta 1 Spectrin
  • Specificityab86184 is specific to the erythroid beta 1 Spectrin.
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Ghost proteins of human red blood cells.

  • General notes


    ab86185 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
    Because the beta 1 Spectrin protein is also present in the membranes of human skeletal muscle cells, ab86185 can also be used to monitor the integrity of muscle cell plasma membranes.

Properties

Applications

Our Abpromise guarantee covers the use of ab86185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 246 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionSpectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
  • Involvement in diseaseDefects in SPTB are the cause of elliptocytosis type 3 (EL3) [MIM:182870]. EL3 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
    Defects in SPTB are the cause of spherocytosis type 2 (SPH2) [MIM:182870]; also known as hereditary spherocytosis type 2 (HS2). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH2 is characterized by severe hemolytic anemia. Inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the spectrin family.
    Contains 2 CH (calponin-homology) domains.
    Contains 17 spectrin repeats.
  • Post-translational
    modifications
    The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta I spectrin antibody
    • Beta spectrin antibody
    • Beta-I spectrin antibody
    • EL3 antibody
    • erythrocyte antibody
    • HS2 antibody
    • HSpTB1 antibody
    • Membrane cytoskeletal protein antibody
    • Spectrin beta antibody
    • Spectrin beta chain antibody
    • Spectrin beta chain erythrocyte antibody
    • Spectrin beta erythrocytic (includes spherocytosis clinical type I) antibody
    • Spectrin beta erythrocytic antibody
    • SPH2 antibody
    • sptB antibody
    • SPTB1 antibody
    • SPTB1_HUMAN antibody
    see all

References for Anti-beta 1 Spectrin antibody [DB2] (ab86185)

ab86185 has not yet been referenced specifically in any publications.

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