Recombinant
RabMAb

Anti-beta 1 Spectrin antibody [EPR6259] (ab129065)

Overview

  • Product name
    Anti-beta 1 Spectrin antibody [EPR6259]
    See all beta 1 Spectrin primary antibodies
  • Description
    Rabbit monoclonal [EPR6259] to beta 1 Spectrin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human beta 1 Spectrin.

  • Positive control
    • Human red blood cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 246 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
    • Involvement in disease
      Defects in SPTB are the cause of elliptocytosis type 3 (EL3) [MIM:182870]. EL3 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
      Defects in SPTB are the cause of spherocytosis type 2 (SPH2) [MIM:182870]; also known as hereditary spherocytosis type 2 (HS2). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH2 is characterized by severe hemolytic anemia. Inheritance is autosomal dominant.
    • Sequence similarities
      Belongs to the spectrin family.
      Contains 2 CH (calponin-homology) domains.
      Contains 17 spectrin repeats.
    • Post-translational
      modifications
      The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cytoplasm > cell cortex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta I spectrin antibody
      • Beta spectrin antibody
      • Beta-I spectrin antibody
      • EL3 antibody
      • erythrocyte antibody
      • HS2 antibody
      • HSpTB1 antibody
      • Membrane cytoskeletal protein antibody
      • Spectrin beta antibody
      • Spectrin beta chain antibody
      • Spectrin beta chain erythrocyte antibody
      • Spectrin beta erythrocytic (includes spherocytosis clinical type I) antibody
      • Spectrin beta erythrocytic antibody
      • SPH2 antibody
      • sptB antibody
      • SPTB1 antibody
      • SPTB1_HUMAN antibody
      see all

    Images

    • Anti-beta 1 Spectrin antibody [EPR6259] (ab129065) at 1/1000 dilution + Red blood cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 246 kDa

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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