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Anti-beta 2 Adrenergic Receptor antibody (ab36956)

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab36956 for help.

Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.

2 questions for ab36956

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Question 1

Tuesday 27-March-2012

I have a question about ab36956, Anti-beta 2 Adrenergic Receptor antibody.

Does ab36956 cross-react with other Adrenergic Receptor protein family members? I purchased ab36956 but having some non-specific like bands in western blotting.

Thank you,

ANSWER:

  

Thank you for your enquiry regarding ab36956.

I've blasted immunogen sequence of ab36956 against human and mouse and in both cases it looks to only detect the beta 2 form. I’ve also blasted the immunogen sequence against the sequence for alpha 1, alpha 2a, alpha 2c, beta 1 and beta 3 forms and I had no matches.

In recent QC test for ab36956, we found blocking with 5% BSA caused very bandy blotting and blocking with 3% milk gave us fine result.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Question 2

Wednesday 11-January-2012

Hello, I'm interested in your antibodies against beta 2 Adrenergic Receptor. I hesitate between the product ab61778 and the product ab36956. I would like to use it for flow cytometry with mouse cells. I would like to check if the receptor reachs the surface of the membrane in mutant cells. Do you know if these antibodies recognize extracellular part of the receptor? I saw on your website pictures of flow cytometry with these antibodies, do you permeabilize cells in these experimentations? Thank you very much.

ANSWER:

  

Thank you for contacting us. Both images were from Abreviews submitted by customers using these products for flow cytometry and they did not permeabilize the membrane before staining.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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