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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Overview
Product name
Anti-beta 2 Adrenergic Receptor antibody
See all beta 2 Adrenergic Receptor products (9) ...
Description
Rabbit polyclonal to beta 2 Adrenergic Receptor
Tested applications
Flow Cyt, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Guinea pig, Chimpanzee, Macaque Monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta 2 Adrenergic Receptor.
(Peptide available as ab38102.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: NIH 3T3 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) and the following tissue lysates: Brain (Human)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Myocardial regeneration
Cardiovascular >> Atherosclerosis >> Ischemia / Reperfusion
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/50
WB: Use a concentration of 1 µg/mlDetects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).Can be blocked with beta 2 Adrenergic Receptor peptide (ab38102).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
Post-translational
modifications
Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP07550
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADRB2 antibody
- ADRB2_HUMAN antibody
- ADRB2R antibody
see all
Database links
- Entrez Gene: 154 Human
- Entrez Gene: 11555 Mouse
- Entrez Gene: 24176 Rat
- Omim: 109690 Human
- SwissProt: P07550 Human
- SwissProt: P18762 Mouse
- SwissProt: P10608 Rat
- Unigene: 2551 Human
see all
Anti-beta 2 Adrenergic Receptor antibody images:
Western blot - beta 2 Adrenergic Receptor antibody (ab36956)
All lanes : Anti-beta 2 Adrenergic Receptor antibody (ab36956) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) with beta 2 Adrenergic Receptor peptide (ab38102) at 1 µg/ml
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179) with beta 2 Adrenergic Receptor peptide (ab38102) at 1 µg/ml
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate with beta 2 Adrenergic Receptor peptide (ab38102) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Flow Cytometry - beta 2 Adrenergic Receptor antibody (ab36956)
ab36956 staining the beta 2 adrenergic receptor in the Human AML cell line Monomac6 by Flow Cytometry. Cells were cultured in RPMI medium supplemented with antibiotics, L-glutamin, minimal essential amino acids and sodium pyruvate in PBS + 1% FCS and 0.01% sodium azide. The sample was incubated with the primary antibody (1/50 in PBS + 1% FCS and 0.01% sodium azide) for 30 minutes at 4°C. A phycoerythrin-conjugated Donkey anti-rabbit IgG (1/100) was used as the secondary antibody.
Gating Strategy: Live Cells.
This image is courtesy of an Abreview submitted by Alexander Kalinkovich
Immunocytochemistry/ Immunofluorescence - beta 2 Adrenergic Receptor antibody (ab36956)
ICC/IF image of ab36956 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36956, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 5µg/ml.
References for Anti-beta 2 Adrenergic Receptor antibody (ab36956)
ab36956 has not yet been referenced specifically in any publications.
Publishing research using ab36956? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"