Anti-beta 2 Adrenergic Receptor antibody (ab40834)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab40834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    modifications
    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Anti-beta 2 Adrenergic Receptor antibody images

  • Anti-beta 2 Adrenergic Receptor antibody (ab40834) at 0.2 µg/ml + Human Liver lysate at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

References for Anti-beta 2 Adrenergic Receptor antibody (ab40834)

This product has been referenced in:
  • Miller EK  et al. Zilpaterol hydrochloride alters abundance of ß-adrenergic receptors in bovine muscle cells but has little effect on de novo fatty acid biosynthesis in bovine subcutaneous adipose tissue explants. J Anim Sci 90:1317-27 (2012). WB ; Cow . Read more (PubMed: 22079997) »
  • Steenhuis P  et al. Adrenergic signaling in human oral keratinocytes and wound repair. J Dent Res 90:186-92 (2011). WB ; Human . Read more (PubMed: 21127260) »

See all 2 Publications for this product

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The host for ab40834 is Goat; you would need an anti goat secondary antibody e.g. ab97100

The host for ab85037 is rabbit; you would need anti rabbit antibody e.g. ab6721

Common secondary can not b...

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Thank you for your enquiry. Ab13989 was tested in Western blot on an E. coli derived fusion protein. The size of the band seen in the Western blot is not typical of endogenous beta 2 andrenergic receptor, which is why the predicted molecular weight...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"