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Products:Microbiology >> Protein >> Human Protein >> Defensin
Overview
Product name
Anti-beta 2 Defensin antibody
See all beta 2 Defensin products (6) ...
Description
Rabbit polyclonal to beta 2 Defensin
Specificity
Human 7 kDa beta 2 Defensin.
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from beta 2 Defensin.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Protein >> Human Protein >> Defensin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/5000.Predicted molecular weight: 7 kDa.
IHC-P: 1/500Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Has antibacterial activity.
Tissue specificity
Expressed in the skin and respiratory tract.
Sequence similarities
Belongs to the beta-defensin family. LAP/TAP subfamily.
Cellular localization
Secreted.
Target information above from: UniProt accessionO15263
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- beta 2 antibody
- BD 2 antibody
- BD-2 antibody
see all
Database links
- Entrez Gene: 100289462 Human
- Entrez Gene: 1673 Human
- Omim: 602215 Human
- SwissProt: O15263 Human
- Unigene: 105924 Human
- Unigene: 721744 Human
Anti-beta 2 Defensin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 2 Defensin antibody (ab63982)
ab63982 (1/500) staining beta 2 defensin in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of a small population of cells within the tonsillar tissue.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 2 Defensin antibody (ab63982)
ab63982 staining beta 2 Defensin in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/500 dilution for 1 hour at 25°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
Image courtesy of Dr Ilka Werner-Martini by Abreview.
References for Anti-beta 2 Defensin antibody (ab63982)
This product has been referenced in:
- Dréno Bet al. Hidradenitis Suppurativa: The Role of Deficient Cutaneous Innate Immunity. Arch Dermatol : (2011). ICC/IF; Human.Read more (PubMed: 22004878) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"