beta 2 Microglobulin Human ELISA Kit (ab108885)
- Product namebeta 2 Microglobulin Human ELISA KitSee all beta 2 Microglobulin kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.7 ng/ml
- Range1 ng/ml - 20 ng/ml
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Beta 2 Microglobulin is a small serum protein that constitutes the light chain of the major histocompatibility class I human leukocyte antigen (HLA class I), an integral membrane protein involved in the immune response. The protein is 99 amino acid residues in length and has molecular mass of 12 kDa. Beta 2 Microglobulin is released from the cell surface of HLA class I into the serum and carried to the kidneys for degradation and secretion. In chronic renal failure, beta 2 Microglobulin accumulates as insoluble amyloid aggregates and causes arthralgias, destructive osteoarthropathies, carpal tunnel syndrome and dialysis-related amyloidosis. Elevated serum beta 2 Microglobulin levels are associated with poor prognosis in multiple myeloma and lymphoma.
ab108885 is designed for detection of human beta 2 Microglobulin in plasma, serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures beta 2 Microglobulin in 4 hours. A polyclonal antibody specific for beta 2 Microglobulin has been pre-coated onto a microplate. Beta 2 Microglobulin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for beta 2 Microglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests beta 2 Microglobulin Microplate 1 unit beta 2 Microglobulin Standard 1 unit Biotinylated beta 2 Microglobulin Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
- B2MBeta 2 microglobulin precursor Beta chain of mhc class 1 proteins Hdcma22p
- SwissProt: Q9UM88 Human
Our Abpromise guarantee covers the use of ab108885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for beta 2 Microglobulin Human ELISA Kit (ab108885)
ab108885 has not yet been referenced specifically in any publications.