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beta 2 Microglobulin Human ELISA Kit (ab108885)

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Overview

  • Product namebeta 2 Microglobulin Human ELISA KitSee all beta 2 Microglobulin kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 0.7 ng/ml
  • Range
    1 ng/ml - 20 ng/ml
  • Recovery

    97.5 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Beta 2 Microglobulin is a small serum protein that constitutes the light chain of the major histocompatibility class I human leukocyte antigen (HLA class I), an integral membrane protein involved in the immune response. The protein is 99 amino acid residues in length and has molecular mass of 12 kDa. Beta 2 Microglobulin is released from the cell surface of HLA class I into the serum and carried to the kidneys for degradation and secretion. In chronic renal failure, beta 2 Microglobulin accumulates as insoluble amyloid aggregates and causes arthralgias, destructive osteoarthropathies, carpal tunnel syndrome and dialysis-related amyloidosis. Elevated serum beta 2 Microglobulin levels are associated with poor prognosis in multiple myeloma and lymphoma.

    ab108885 is designed for detection of human beta 2 Microglobulin in plasma, serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures beta 2 Microglobulin in 4 hours. A polyclonal antibody specific for beta 2 Microglobulin has been pre-coated onto a microplate. Beta 2 Microglobulin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for beta 2 Microglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceBeta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
    • Alternative names
        B2MBeta 2 microglobulin precursor Beta chain of mhc class 1 proteins Hdcma22p
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108885 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      beta 2 Microglobulin Human ELISA Kit images

      • Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit - 1 x 96 Wells Plate (ab108885)
        Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit - 1 x 96 Wells Plate (ab108885)

      Protocols

      References for beta 2 Microglobulin Human ELISA Kit (ab108885)

      ab108885 has not yet been referenced specifically in any publications.

      Publishing research using ab108885 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108885 Submit a Question for ab108885

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      Would you be able to provide expiry dates for current stock please?



      We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (http://www.abcam.com/index.ht...

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      we have a customer that would be interested in testing ab108885 and ab99977 in sampes of human liquor.

      Do you accept this proposal for applying these two abreviews?

      Thanks in advance and kind regards

      Ab108885
      DISCOUNT CODE: xxxxx
      Expiration date:xxxxxxx

      Ab99977
      DISCOUNT CODE:xxxxxxxxx
      Expiration date: xxxxxxxx

      I am very pleased to hear that your customer would like to test ab99977 and ab108885 in human liquor. ...

      Read More

      Good morning,
      I would like to receive some technical information and a quote for your product:

      Beta 2 microglobulin human ELISA Kit

      I need to find the beta 2-microglobulin in plasma samples from fetal blood.
      The test I need...

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      Thank you for your enquiry and your interest in our products.

      Currently, we do have 2 kits for detecting human beta 2 Microglobulin i.e. ab99977 and ab108885.

      I would advise you to read through the datasheets as well as the Protocol Bo...

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      Customer kindly called to discuss using our beta 2 Microglobulin products in ELISA on porcine tissues.

      Thank you for contacting Abcam. I have been in contact with the labs about our kit and also have some additional details regarding our other Beta-2-Microglobulin products reactivity with porcine.

      For our kits:

      Regardingab99977:Unfort...

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      Dear Sir/madam,

      I am planning to buy 3 * 3 kits i.e 9 kits in total for detecting 3 different things as following:


      Albumin Human ELISA Kit

      Transferrin Human ELISA Kit

      beta 2 Microglobulin Human ELISA Kit <...

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      Thank you for your inquiry and you positive feedback.

      Please see my answers to your questions below:

      1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially optimis...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"