beta 2 Microglobulin Human ELISA Kit (ab108885)

Overview

  • Product namebeta 2 Microglobulin Human ELISA Kit
    See all beta 2 Microglobulin kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.19 ng/ml
  • Range
    1 ng/ml - 20 ng/ml
  • Recovery

    97 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s beta 2 Microglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of beta 2 Microglobulin levels in plasma, serum, milk, saliva, urine, CSF and cell culture supernatants.

    A beta 2 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a beta 2 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of beta 2 Microglobulin captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceBeta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
  • Cellular localizationSecreted
  • Alternative names
    • B2M
    • Beta 2 microglobulin precursor
    • Beta chain of mhc class 1 proteins
    • Hdcma22p
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

beta 2 Microglobulin Human ELISA Kit images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (mU) per mL of tested sample

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample

Protocols

References for beta 2 Microglobulin Human ELISA Kit (ab108885)

ab108885 has not yet been referenced specifically in any publications.

Product Wall



We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (http://www.abcam.com/index...

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Ab108885
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Ab99977
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I am very pleased to hear that your customer would like to test ab99977 and ab108885 in human liquo...

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Thank you for your enquiry and your interest in our products.

Currently, we do have 2 kits for detecting human beta 2 Microglobulin i.e. ab99977 and ab108885.

I would advise you to read through the datasheets as well as the Protocol...

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Thank you for contacting Abcam. I have been in contact with the labs about our kit and also have some additional details regarding our other Beta-2-Microglobulin products reactivity with porcine.

For our kits:

Regardingab99977:Unf...

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Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially opti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"