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- Secondary antibodies
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Product name
Anti-beta 2 Microglobulin antibody [B2M-01] (Phycoerythrin)
See all beta 2 Microglobulin products (28) ...
Description
Mouse monoclonal [B2M-01] to beta 2 Microglobulin (Phycoerythrin)
Conjugation
Phycoerythrin
Conjugation notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
This antibody reacts with beta 2 microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 microglobulin. Beta 2 mircoglobulin is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rabbit, Chicken, Cow, Dog
Immunogen
Full length native protein (purified) (Human)
Positive control
Peripheral blood lymphocytes.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.2% BSA, PBS
Concentration
Concentration information loading...
Purity
Size exclusion
Clonality
Monoclonal
Clone number
B2M-01
Isotype
IgG2a
Research areas
Cardiovascular >> Blood >> Blood Cell Antigens >> WBC Antigens >> HLA
Cancer >> Tumor biomarkers >> Globulins
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use a concentration of 5 µg/ml.
Target
Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Post-translational
modifications
Glycation of Ile-21 is observed in long-term hemodialysis patients.
Cellular localization
Secreted. Detected in serum and urine.
Target information above from: UniProt accessionP61769
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobulin precursor antibody
see all
Database links
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
References for Anti-beta 2 Microglobulin antibody [B2M-01] (Phycoerythrin) (ab49424)
This product has been referenced in:
- Khurana M et al. Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol 21:1257-65 (2006).Read more (PubMed: 16810512) »
- Hilgert I et al. The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) 30:369-76 (1984).Read more (PubMed: 6394369) »
See all 2 publications for this product
Publishing research using ab49424? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"