 image](#beta-2-Microglobulin-Primary-antibodies-ab27588-2.jpg)
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Product name
Anti-beta 2 Microglobulin antibody [B2M-02]
See all beta 2 Microglobulin products (28) ...
Description
Mouse monoclonal [B2M-02] to beta 2 Microglobulin
Conjugation notes
Ab27588 is free of unconjugated biotin.
Specificity
Ab27588 reacts with beta 2 Microglobulin associated with cell surface MHC Class I molecules and other membrane antigens as well as with soluble beta2 microglobulin.
Tested applications
Flow Cyt, IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human, Pig
Immunogen
Purified isolated human beta 2 Microglobulin.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide.
Constituents: PBS, pH 7.4.
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Ab27588 was purified from ascites by affinity chromatography. > 95% (by SDS PAGE)
Clonality
Monoclonal
Clone number
B2M-02
Isotype
IgG2a
Research areas
Cardiovascular >> Blood >> Blood Cell Antigens >> WBC Antigens >> HLA
Cancer >> Tumor biomarkers >> Globulins
Cardiovascular >> Blood >> Serum Proteins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-beta 2 Microglobulin antibody [B2M-02](ab27588)
 image](/ps/datasheet/images/27/ab27588/beta-2-Microglobulin-Primary-antibodies-ab27588-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab27588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
- 1 Image
IHC-P
ELISA
WB
Application notes
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6.0 before commencing with IHC staining protocol.
WB: Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Post-translational
modifications
Glycation of Ile-21 is observed in long-term hemodialysis patients.
Cellular localization
Secreted. Detected in serum and urine.
Target information above from: UniProt accessionP61769
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobulin precursor antibody
see all
Database links
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- SwissProt: Q07717 Pig
- Unigene: 534255 Human
- Unigene: 702947 Human
Anti-beta 2 Microglobulin antibody [B2M-02] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-beta 2 Microglobulin antibody [B2M-02](ab27588)
Ab27588 staining human normal skin tissue. Staining is localised to cell membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-beta 2 Microglobulin antibody [B2M-02] (ab27588)
This product has been referenced in:
- Drbal K et al. A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood 98:1561-6 (2001).Read more (PubMed: 11520808) »
- Hilgert I et al. The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) 30:369-76 (1984).Read more (PubMed: 6394369) »
See all 2 publications for this product
Publishing research using ab27588? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"