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Western blot - beta 2 Microglobulin antibody [EP2978Y] (ab75853)
![Western blot - beta 2 Microglobulin antibody [EP2978Y] (ab75853)](/ps/datasheet/images/ab75853wb.jpg)
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Product Name
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beta 2 Microglobulin antibody [EP2978Y]
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See all beta 2 Microglobulin antibodies (30)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP2978Y] to beta 2 Microglobulin
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Immunogen
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A synthetic peptide corresponding to residues on the C-terminus of human beta 2 Microglobulin.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/60.
ICC: 1/100 - 1/250.
IP: 1/40.
WB: 1/100000 - 1/200000. Predicted molecular weight: 14 kDa.
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Raji, U937, HeLa and human bone marrow lysates
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Cellular localization
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Secreted
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Research areas
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Cardiovascular >> Blood >> Blood Cell Antigens >> WBC Antigens >> HLA
Cancer >> Tumor biomarkers >> Globulins
Cardiovascular >> Blood >> Serum Proteins
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Relevance
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Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP2978Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta 2 Microglobulin antibody [EP2978Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for beta 2 Microglobulin antibody [EP2978Y] images, references, products related to ab75853 and other tools.
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beta 2 Microglobulin antibody [EP2978Y] images:
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 Western blot - beta 2 Microglobulin antibody [EP2978Y] (ab75853)
All lanes : beta 2 Microglobulin antibody [EP2978Y] (ab75853) at 1/200000 dilution
Lane 1 : Raji cell lysate
Lane 2 : U937 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human bone marrow lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size : 14 kDa
Observed band size : 14 kDa
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Search PubMed (MEDLINE) for references to beta 2 Microglobulin
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beta 2 Microglobulin antibody [EP2978Y] - more information
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Customer reviews (feedback) regarding beta 2 Microglobulin antibody [EP2978Y] |
Customer FAQs regarding beta 2 Microglobulin antibody [EP2978Y] |
Protocols for beta 2 Microglobulin antibody [EP2978Y] |
Price and availability of products related to beta 2 Microglobulin antibody [EP2978Y] |
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beta 2 Microglobulin antibody [EP2978Y] - related products:
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beta 2 Microglobulin antibody [EP2978Y] - other tools:
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Contact Abcam with a Technical Enquiry about beta 2 Microglobulin antibody [EP2978Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - beta 2 Microglobulin antibody [EP2978Y] (ab75853)](/index.html?datasheet=75853#ab75853wb.jpg)
