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Anti-beta 2 Microglobulin antibody [EP2978Y] (ab75853)

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Overview

Product name

Anti-beta 2 Microglobulin antibody [EP2978Y]
See all beta 2 Microglobulin products (28) ...

Description

Rabbit monoclonal [EP2978Y] to beta 2 Microglobulin

Tested applications

WB, IP, ICC, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues on the C-terminus of human beta 2 Microglobulin.

Positive control

Raji, U937, HeLa and human bone marrow lysates

General notes

Produced under U.S. Patent No. 5,675,063

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP2978Y

Isotype

IgG

Research areas

Cardiovascular >> Blood >> Blood Cell Antigens >> WBC Antigens >> HLA
Cancer >> Tumor biomarkers >> Globulins
Cardiovascular >> Blood >> Serum Proteins

  • Western blot - beta 2 Microglobulin antibody [EP2978Y] (ab75853)Western blot - beta 2 Microglobulin antibody [EP2978Y] (ab75853) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide2 Images

    WB

    Three stars (3 Abreviews) WB: 1/10000 - 1/50000.Pr...Read more →

    WB: 1/10000 - 1/50000.Predicted molecular weight: 14 kDa.(This antibody has been found to work at dilutions up to 1:200,000 in WB.)

  • IP

     IP: 1/40

    IP: 1/40

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

  • Flow Cyt

     Flow Cyt: 1/60

    Flow Cyt: 1/60

Application notes

Is unsuitable for or IHC-P.

Target

Function

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

Involvement in disease

Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

Sequence similarities

Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Post-translational
modifications

Glycation of Ile-21 is observed in long-term hemodialysis patients.

Cellular localization

Secreted. Detected in serum and urine.

Target information above from: UniProt accessionP61769 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • B2M antibody
  • B2MG_HUMAN antibody
  • Beta 2 microglobulin precursor antibody
  • Beta chain of mhc class 1 proteins antibody
  • Beta-2-microglobulin form pI 5.3 antibody
  • Hdcma22p antibody
see all

Database links

see all

Anti-beta 2 Microglobulin antibody [EP2978Y] images:

  Western blot - beta 2 Microglobulin antibody [EP2978Y] (ab75853)

Western blot - beta 2 Microglobulin antibody [EP2978Y] (ab75853)

All lanes : Anti-beta 2 Microglobulin antibody [EP2978Y] (ab75853) at 1/200000 dilution

Lane 1 : Raji cell lysate
Lane 2 : U937 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human bone marrow lysate

Lysates/proteins at 10 µg per lane.

Secondary
HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size : 14 kDa
Observed band size : 14 kDa

References for Anti-beta 2 Microglobulin antibody [EP2978Y] (ab75853)

ab75853 has not yet been referenced specifically in any publications.

Publishing research using ab75853? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"