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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab15976? Please let us know so that we can cite the reference in this datasheet
ab15976 has been referenced in 3 publications.
- Martins R et al. Differential HFE gene expression is regulated by alternative splicing in human tissues. PLoS One 6:e17542 (2011). WB, ICC/IF; Human. PubMed: 21407826
- Bhatt L et al. Knockdown of beta2-microglobulin perturbs the subcellular distribution of HFE and hepcidin. Biochem Biophys Res Commun 378:727-31 (2009). ICC/IF; Human. PubMed: 19059216
- Bhatt L et al. The hereditary hemochromatosis protein HFE and its chaperone beta2-microglobulin localise predominantly to the endosomal-recycling compartment. Biochem Biophys Res Commun 359:277-84 (2007). PubMed: 17543888
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 2 Microglobulin antibody (ab15976)
Ab15976 staining Human normal skin. Staining is localized to cell membrane (and to lesser amount cytoplasmatic compartment).
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot
Anti-beta 2 Microglobulin antibody (ab15976) at 1 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
beta 2 Microglobulin antibody for ICC/IF in Human (15976)
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