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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-beta 2 Microglobulin antibody
See all beta 2 Microglobulin products (28) ...
Description
Rabbit polyclonal to beta 2 Microglobulin
Specificity
The antibody reacts with human beta 2 microglobulin. The specificity of the antibody has been ascertained by crossed immunoelectrophoresis.
Tested applications
ELISA, IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Beta-2-microglobulin isolated from the urine of patients with tubular proteinuria.
Positive control
IHC-P: human liver, human skin
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.1mM Sodium chloride
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This "Immunoglobulin Fraction" is purified by four purification steps: A) Ammonium sulphate precipitation, B) Lipoprotein dialysis to remove lipoproteins, C) DEAE-anion exchange chromatography, and D) a second ammonium sulphate precipitaiton. The purified immunoglobulin fraction is then "Specificity Purified" by solid phase absorption which serves to remove possible trace amounts of unspecific immunoglobulins from the antibody. A solid phase absorption is performed by passing the antibody through a Sepharose solid matrix coupled with human serum proteins that bind the unspecific immunoglobulins in the antibody by an antigen-antibody reaction. This process ensures that the antibody is specific (i.e. it reacts only with beta 2 Microglobulin and no other human proteins).
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Blood Cell Antigens >> WBC Antigens >> HLA
Cancer >> Tumor biomarkers >> Globulins
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 10 µg/ml
IHC-P: 1/400 - 1/800.Do not perform antigen retrieval.
WB: Use at an assay dependent dilution. Predicted molecular weight: 13 kDa.
ICC/IF: 1/200(See Abreview.)
Target
Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Post-translational
modifications
Glycation of Ile-21 is observed in long-term hemodialysis patients.
Cellular localization
Secreted. Detected in serum and urine.
Target information above from: UniProt accessionP61769
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobulin precursor antibody
see all
Database links
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
Anti-beta 2 Microglobulin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 2 Microglobulin antibody (ab15976)
Ab15976 staining Human normal skin. Staining is localized to cell membrane (and to lesser amount cytoplasmatic compartment).
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot
Anti-beta 2 Microglobulin antibody (ab15976) at 1 µg/ml + beta 2 Microglobulin protein (Human) (ab73694) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
References for Anti-beta 2 Microglobulin antibody (ab15976)
This product has been referenced in:
- Martins Ret al. Differential HFE gene expression is regulated by alternative splicing in human tissues. PLoS One 6:e17542 (2011). WB, ICC/IF; Human.Read more (PubMed: 21407826) »
- Bhatt Let al. Knockdown of beta2-microglobulin perturbs the subcellular distribution of HFE and hepcidin. Biochem Biophys Res Commun 378:727-31 (2009). ICC/IF; Human.Read more (PubMed: 19059216) »
See all 3 publications for this product
Publishing research using ab15976? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"