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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 2 Microglobulin antibody (ab15976)
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Product Name
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beta 2 Microglobulin antibody
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See all beta 2 Microglobulin antibodies (30)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to beta 2 Microglobulin
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Immunogen
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Beta-2-microglobulin isolated from the urine of patients with tubular proteinuria.
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Reacts with
(species key)
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Hu
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Specificity
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The antibody reacts with human beta 2 microglobulin. The specificity of the antibody has been ascertained by crossed immunoelectrophoresis.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 10 µg/ml.
ICC/IF: 1/200 (see Abreview).
IHC-P: 1/400 - 1/800 (30 min at RT, when used with Envision/HRP Kit). Pretreatment of tissues with heat-induced epitope retrieval is not required.
WB: Use at an assay dependent dilution. Predicted molecular weight: 13 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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IHC-P: human liver, human skin
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Cellular localization
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Secreted
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Research areas
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Cardiovascular >> Blood >> Blood Cell Antigens >> WBC Antigens >> HLA
Cancer >> Tumor biomarkers >> Globulins
Cardiovascular >> Blood >> Serum Proteins
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Relevance
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Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.1mM Sodium chloride Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This "Immunoglobulin Fraction" is purified by four purification steps: A) Ammonium sulphate precipitation, B) Lipoprotein dialysis to remove lipoproteins, C) DEAE-anion exchange chromatography, and D) a second ammonium sulphate precipitaiton.
The purified immunoglobulin fraction is then "Specificity Purified" by solid phase absorption which serves to remove possible trace amounts of unspecific immunoglobulins from the antibody. A solid phase absorption is performed by passing the antibody through a Sepharose solid matrix coupled with human serum proteins that bind the unspecific immunoglobulins in the antibody by an antigen-antibody reaction. This process ensures that the antibody is specific (i.e. it reacts only with beta 2 Microglobulin and no other human proteins).
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Form
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Liquid
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Concentration
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10.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta 2 Microglobulin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for beta 2 Microglobulin antibody images, references, products related to ab15976 and other tools.
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beta 2 Microglobulin antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 2 Microglobulin antibody (ab15976)
Ab15976 staining Human normal skin. Staining is localized to cell membrane (and to lesser amount cytoplasmatic compartment).
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for beta 2 Microglobulin antibody (ab15976)
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This product has been used in:
Bhatt L et al. The hereditary hemochromatosis protein HFE and its chaperone beta2-microglobulin localise predominantly to the endosomal-recycling compartment. Biochem Biophys Res Commun 359:277-84 (2007). PubMed: 17543888
If you publish research using ab15976 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to beta 2 Microglobulin
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